{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T17:50:33Z","timestamp":1781286633049,"version":"3.54.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The occurrence of 36 environmentally regulated genes of\n            <jats:italic>Streptococcus suis<\/jats:italic>\n            strain 10 among all 35\n            <jats:italic>S. suis<\/jats:italic>\n            serotypes was determined by using hybridization with the amplified genes as probes. In addition, the distribution of these genes among the virulence phenotypes of serotypes 1 and 2 was assessed. Hybridization was also performed with various other streptococcal species and nonstreptococcal bacterial species which may be present in pigs. Interestingly, probe ivs-25\/iri-1, similar to\n            <jats:italic>agrA<\/jats:italic>\n            and\n            <jats:italic>sapR<\/jats:italic>\n            , hybridized only with\n            <jats:italic>S. suis<\/jats:italic>\n            serotype 1 and 2 strains with virulent phenotypes and is therefore suitable as a diagnostic parameter. Only one probe was specific for\n            <jats:italic>S. suis<\/jats:italic>\n            . This probe's sequence was identical to the\n            <jats:italic>epf<\/jats:italic>\n            gene, a putative virulence factor of\n            <jats:italic>S. suis<\/jats:italic>\n            . Probe ivs-31 was similar to a virulence factor of\n            <jats:italic>S. suis<\/jats:italic>\n            , namely, a gene encoding a fibronectin- and fibrinogen-binding protein. This probe hybridized only with oral streptococci. Nearly half of the probes (45%) hybridized with the oral streptococci (\n            <jats:italic>S. oralis<\/jats:italic>\n            ,\n            <jats:italic>S. milleri<\/jats:italic>\n            ,\n            <jats:italic>S. sanguis<\/jats:italic>\n            ,\n            <jats:italic>S. gordonii<\/jats:italic>\n            , and\n            <jats:italic>S. mitis<\/jats:italic>\n            ) and with\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            . This indicates a close relationship between\n            <jats:italic>S. suis<\/jats:italic>\n            , the oral streptococci, and\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            with respect to the selected environmentally regulated genes. One probe only hybridized with gram-negative species and therefore seems to be obtained by\n            <jats:italic>S. suis<\/jats:italic>\n            from a gram-negative organism by horizontal transfer.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.9.3261-3268.2002","type":"journal-article","created":{"date-parts":[[2002,8,29]],"date-time":"2002-08-29T19:27:50Z","timestamp":1030649270000},"page":"3261-3268","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Distribution of Environmentally Regulated Genes of\n            <i>Streptococcus suis<\/i>\n            Serotype 2 among\n            <i>S. suis<\/i>\n            Serotypes and Other Organisms"],"prefix":"10.1128","volume":"40","author":[{"given":"Astrid","family":"de Greeff","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1100 DD, Amsterdam"},{"name":"Division of Infectious Diseases and Food Chain Quality, Cluster of Endemic Diseases, Institute for Animal Science and Health, 8200 AB Lelystad"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herma","family":"Buys","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Food Chain Quality, Cluster of Endemic Diseases, Institute for Animal Science and Health, 8200 AB Lelystad"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Verhaar","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Food Chain Quality, Cluster of Endemic Diseases, Institute for Animal Science and Health, 8200 AB Lelystad"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loek","family":"van Alphen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, 1100 DD, Amsterdam"},{"name":"Laboratory for Vaccine Research, RIVM, National Institute of Public Health and the Environment, 3720 BA Bilthoven, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilde E.","family":"Smith","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Food Chain Quality, Cluster of Endemic Diseases, Institute for Animal Science and Health, 8200 AB Lelystad"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.10.5376-5385.1989"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.20.5.945-947.1984"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.1.131"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02218.x"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.10.4828-4833.2001"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.2.362-366.1999"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(17)30581-X"},{"key":"e_1_3_1_9_2","unstructured":"Clifton-Hadley F. 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In P. G. Monetti and G. Vignola (ed.) Proceedings of the 14th International Pig Veterinary Society Congress. 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