{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:39:11Z","timestamp":1760027951754},"reference-count":27,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Broth culture of\n            <jats:italic>Trichomonas vaginalis<\/jats:italic>\n            is considered the \u201cgold standard\u201d for the diagnosis of trichomoniasis. Two studies were carried out to evaluate modified Columbia agar (MCA) for the isolation of\n            <jats:italic>T. vaginalis<\/jats:italic>\n            from clinical samples. Study I compared isolation on MCA to that on liquid medium with 889 vaginal samples. Out of 63 samples positive for\n            <jats:italic>T. vaginalis<\/jats:italic>\n            (7.1% of total), MCA identified 62 (98.4%) and broth identified 58 (92.1%). In study II, trichomoniasis was diagnosed within the scope of a screening program for a total of 39,585 men and women by culture on MCA and direct microscopy. Culture on MCA detected 199 (98.5%) and Gram staining detected 163 (80.7%) of 202 positive specimens. Wet-mount preparations used for symptomatic patients identified 103 (92.8%) of 111 cases. Culture of\n            <jats:italic>T. vaginalis<\/jats:italic>\n            from clinical samples on MCA is highly sensitive and reliable, as well as timesaving, and therefore suitable for screening of symptomatic and asymptomatic individuals.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.9.3277-3280.2002","type":"journal-article","created":{"date-parts":[[2002,8,29]],"date-time":"2002-08-29T19:27:50Z","timestamp":1030649270000},"page":"3277-3280","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Detection of\n            <i>Trichomonas vaginalis<\/i>\n            on Modified Columbia Agar in the Routine Laboratory"],"prefix":"10.1128","volume":"40","author":[{"given":"Angelika","family":"Stary","sequence":"first","affiliation":[{"name":"Outpatients' Center for Diagnosis of Infectious Venerodermatological Diseases, A-1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelika","family":"Kuchinka-Koch","sequence":"additional","affiliation":[{"name":"Outpatients' Center for Diagnosis of Infectious Venerodermatological Diseases, A-1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilianna","family":"Teodorowicz","sequence":"additional","affiliation":[{"name":"Outpatients' Center for Diagnosis of Infectious Venerodermatological Diseases, A-1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"149","volume":"67","year":"1991","unstructured":"Borchardt, K. 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Orsi. 1958. Cultivation of Trichomonas vaginalis on a solid medium and its application to the assay of trichomycin potency. Antibiot. Chemother.8:561-563.","journal-title":"Antibiot. Chemother."},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/141.2.137"},{"key":"e_1_3_1_10_2","first-page":"116","volume":"14","year":"2001","unstructured":"Garland, S. M. 2001. Trichomonas vaginalis: why we should be screening. Venereology14:116-120.","journal-title":"Venereology"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.22.3.269"},{"key":"e_1_3_1_12_2","first-page":"93","volume":"52","year":"1995","unstructured":"Hill, W. M. 1995. Routine detection of Trichomonas vaginalis in genital specimens using culture in microtitre trays. Br. J. Biomed. Sci.52:93-96.","journal-title":"Br. J. Biomed. 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Errors in the diagnosis of Trichomonas vaginalis infections as observed among 1199 patients. Obstet. Gynecol.39:7-9.","journal-title":"Obstet. Gynecol."},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.2.300"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.2.304"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.10.2630-2631.1996"},{"key":"e_1_3_1_27_2","unstructured":"Rein M. F. and M. M\u00fcller. 1990. Trichomonas vaginalis and trichomoniasis p. 481-492. In K. K. Holmes P.-A. M\u00e5rdh P. F. Sparling and P. J. Wiesner (ed.) Sexually transmitted diseases. McGraw-Hill Inc. 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