{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:28:33Z","timestamp":1787941713512,"version":"build-2784847793"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The aim of the present study was to evaluate the accuracy of the new VITEK 2 system (bioMe\u0301rieux, Marcy l' Etoile, France) for the detection of methicillin resistance in coagulase-negative staphylococci (CoNS) by using AST-P515 and AST-P523 test cards. Analyses of the VITEK 2 oxacillin MIC determination evaluated according to the actual breakpoint (\u22650.5 \u03bcg\/ml) of the National Committee for Clinical Laboratory Standards resulted in a high sensitivity of 99.2% but a moderate specificity of 80%. The newly included oxacillin resistance (OR) test of the VITEK 2 system displayed a high sensitivity and a high specificity of 97.5 and 98.7%, respectively. Concordance between the results of the\n            <jats:italic>mecA<\/jats:italic>\n            PCR and the VITEK 2 oxacillin MIC was observed for almost all\n            <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n            strains, but the reduced specificity was attributable to higher oxacillin MICs for\n            <jats:italic>mecA<\/jats:italic>\n            -negative non-\n            <jats:italic>S. epidermidis<\/jats:italic>\n            strains, especially\n            <jats:italic>S. saprophyticus<\/jats:italic>\n            ,\n            <jats:italic>S. lugdunensis<\/jats:italic>\n            , and\n            <jats:italic>S. cohnii<\/jats:italic>\n            . Evaluation of alternative oxacillin MIC breakpoints of 1, 2, or 4 \u03bcg\/ml resulted in improved degrees of specificity of 84, 90.7, and 97.3%, respectively. Only minor changes occurred in the corresponding sensitivity values, which were 98.4, 97.5, and 97.5%, respectively. Methicillin resistance in CoNS was detected after 7 and 8 h in 91.1 and 93.5% of the\n            <jats:italic>mecA<\/jats:italic>\n            -positive strains, respectively, by the VITEK 2 OR test and in 86.3 and 89.5% of the\n            <jats:italic>mecA<\/jats:italic>\n            -positive strains, respectively, by VITEK 2 oxacillin MIC determination. After 7 and 8 h the VITEK 2 OR test classified 59.2 and 78.9% of the\n            <jats:italic>mecA<\/jats:italic>\n            -negative strains, respectively, as susceptible to oxacillin, whereas comparable values were obtained 2 h later by VITEK 2 oxacillin MIC determination. The results of our study encourage the use of the VITEK 2 system, which proved to be a highly reliable and rapid phenotypic method for the detection of methicillin resistance in CoNS.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.9.3291-3295.2002","type":"journal-article","created":{"date-parts":[[2002,8,29]],"date-time":"2002-08-29T19:27:50Z","timestamp":1030649270000},"page":"3291-3295","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci with the VITEK 2 System"],"prefix":"10.1128","volume":"40","author":[{"given":"Matthias A.","family":"Horstkotte","sequence":"first","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, D-20246 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes K.-M.","family":"Knobloch","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, D-20246 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Rohde","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, D-20246 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Dobinsky","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, D-20246 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietrich","family":"Mack","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, D-20246 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/45.2.259"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.10.2231"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.5.1415-1418.1999"},{"key":"e_1_3_2_5_2","unstructured":"Berger-B\u00e4chi B. 1997. 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