{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,8]],"date-time":"2026-11-08T03:50:51Z","timestamp":1794109851690,"version":"4.8.0"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A novel commercially available enzyme-linked immunosorbent assay (ELISA) for prevaccination screening and diagnosis of Q fever (PanBio\n            <jats:italic>Coxiella burnetii<\/jats:italic>\n            immunoglobulin G [IgG] ELISA) was compared to the complement fixation test (CFT), and the indirect fluorescent-antibody test (IFAT) was used to resolve discrepant results between the other two tests. A total of 214 serum samples was tested. The ELISA demonstrated a specificity of 96% (46 of 48 samples) and a sensitivity of 71% (95 of 134 samples). Of the six serum pairs showing CFT seroconversion, three pairs showed a corresponding ELISA seroconversion. No cross-reactivity was observed in the ELISA with serum samples from patients with mycoplasma, brucella, and chlamydia infections. One of the 13 patients with leptospirosis demonstrated a positive result in the ELISA but not in the CFT or the IFAT, and\n            <jats:italic>Legionella pneumophila<\/jats:italic>\n            serogroup 4 antibody was found in one of the two sera that were false-positive by ELISA. The results presented in this study suggest that the PanBio Q fever IgG ELISA is a specific alternative method for prevaccination testing and an aid for the diagnosis of Q fever. This test is suitable for use as a screening assay, with CFT and\/or IFAT used to confirm negative results.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.9.3526-3529.2002","type":"journal-article","created":{"date-parts":[[2002,8,29]],"date-time":"2002-08-29T19:27:50Z","timestamp":1030649270000},"page":"3526-3529","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Evaluation of a Novel Commercial Enzyme-Linked Immunosorbent Assay Detecting\n            <i>Coxiella burnetii<\/i>\n            -Specific Immunoglobulin G for Q Fever Prevaccination Screening and Diagnosis"],"prefix":"10.1128","volume":"40","author":[{"given":"Peter R.","family":"Field","sequence":"first","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avelina","family":"Santiago","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sau-Wan","family":"Chan","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhara B.","family":"Patel","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Dickeson","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jody L.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"PanBio Pty Limited, Brisbane, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter L.","family":"Devine","sequence":"additional","affiliation":[{"name":"PanBio Pty Limited, Brisbane, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan M.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Viral Diagnostic and Referral Laboratory, North Ryde, New South Wales"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(88)90087-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01691965"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/148.3.477"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.4.1645-1647.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.7.1823-1834.1998"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(95)00121-P"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.10.3380-3387.1990"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.39.2.977-981.1983"},{"key":"e_1_3_2_10_2","unstructured":"Marmion B. P. 1999. Q fever: your questions answered. CSL Ltd. Melbourne Victoria Australia."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-2998(78)80014-2"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1970.tb84481.x"},{"key":"e_1_3_2_13_2","first-page":"391","volume":"12","year":"1980","unstructured":"Murphy, A. M., and L. Magro. 1980. IgM globulin response in Q fever (Coxiella burnetii) infections. Pathology12:391-396.","journal-title":"Pathology"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02112627"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.6.1063-1067.1987"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.1938-1942.1997"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-19-3-281"},{"key":"e_1_3_2_18_2","first-page":"61","volume":"13","year":"1997","unstructured":"Xu, H., J. Lohr, and M. Greiner. 1997. The selection of ELISA cut-off points for testing antibody to Newcastle disease by two-graph receiver-operating characteristic (TG-ROC) analysis. J. Immunol. Methods13:61-64.","journal-title":"J. Immunol. 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