{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:51:31Z","timestamp":1790232691683,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A\n            <jats:italic>Blastomyces dermatitidis<\/jats:italic>\n            nested PCR assay targeting the gene encoding the Wisconsin 1 (WI-1) adhesin was developed and compared with a nested PCR targeting the 18S rRNA gene (rDNA) of members of the family\n            <jats:italic>Onygenaceae<\/jats:italic>\n            . We examined 73 paraffin-embedded tissue samples obtained from nine dogs which died of blastomycosis and nine dogs which succumbed to lymphosarcoma according to autopsy findings; amplifiable canine DNA was extracted from 25 and 33 specimens from the two groups, respectively. The\n            <jats:italic>B. dermatitidis<\/jats:italic>\n            PCR amplified DNA from 8 of 13 tissue samples in which yeast cells were detected by microscopy. Sequencing revealed that all PCR products were homologous to the\n            <jats:italic>B. dermatitidis<\/jats:italic>\n            WI-1 adhesin gene. No PCR product was amplified from 12 microscopically negative biopsy specimens from dogs with blastomycosis or from 33 biopsy specimens from dogs with lymphosarcoma. The 18S rDNA PCR amplified DNA from 10 and 9 tissue samples taken from dogs which died of blastomycosis and lymphosarcoma, respectively. Only six products were identified as being identical to\n            <jats:italic>B. dermatitidis<\/jats:italic>\n            18S rDNA; they were exclusively obtained from specimens positive by the\n            <jats:italic>B. dermatitidis<\/jats:italic>\n            nested PCR. For specificity testing, 20 human biopsy specimens proven to have histoplasmosis were examined, and a specific\n            <jats:italic>H. capsulatum<\/jats:italic>\n            product was amplified by the 18S rDNA PCR from all specimens, whereas no product was obtained from any of the 20 samples by the\n            <jats:italic>B. dermatitidis<\/jats:italic>\n            PCR assay. In conclusion, the PCR targeting a gene encoding the unique WI-1 adhesin is as sensitive as but more specific than the PCR targeting the 18S rDNA for detection of\n            <jats:italic>B. dermatitidis<\/jats:italic>\n            in canine tissue.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.1.205-208.2003","type":"journal-article","created":{"date-parts":[[2003,1,7]],"date-time":"2003-01-07T18:03:11Z","timestamp":1041962591000},"page":"205-208","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Nested PCR Assays for Detection of\n            <i>Blastomyces dermatitidis<\/i>\n            DNA in Paraffin-Embedded Canine Tissue"],"prefix":"10.1128","volume":"41","author":[{"given":"Ralf","family":"Bialek","sequence":"first","affiliation":[{"name":"Institute for Tropical Medicine, University Hospital Tu\u0308bingen, Tu\u0308bingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Cascante Cirera","sequence":"additional","affiliation":[{"name":"Institute for Tropical Medicine, University Hospital Tu\u0308bingen, Tu\u0308bingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanja","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Institute for Tropical Medicine, University Hospital Tu\u0308bingen, Tu\u0308bingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Aepinus","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology, University of Wu\u0308rzburg, Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie I.","family":"Shearn-Bochsler","sequence":"additional","affiliation":[{"name":"Department of Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred M.","family":"Legendre","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, University of Tennessee, Knoxville, Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219580000361"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/39.3.1704393"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.8.2940-2942.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.9.3190-3193.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1506-1509.2001"},{"key":"e_1_3_2_7_2","first-page":"463","volume":"9","year":"2002","unstructured":"Bialek, R., M. Weiss, K. Bekure-Nemariam, L. K. Najvar, M. B. Alberdi, J. R. Graybill, and U. Reischl. 2002. Detection of Cryptococcus neoformans DNA in tissue samples by nested and real-time PCR assays. Clin. Diagn. Lab. Immunol.9:463-471.","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.5.1644-1647.2002"},{"key":"e_1_3_2_9_2","first-page":"15335","volume":"163","year":"1988","unstructured":"Ercolani, L., B. Florence, M. Denaro, and M. Alexander. 1988. Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene. J. Biol. Chem.163:15335-15341.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a121184"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-200103000-00004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/315435"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/168.3.779"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2000.61.554"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(00)05007-5"},{"key":"e_1_3_2_16_2","first-page":"2109","volume":"41","year":"1980","unstructured":"Legendre, A. M., and P. U. Becker. 1980. Evaluation of the agar-gel immunodiffusion test in the diagnosis of canine blastomycosis. Am. J. Vet. Res.41:2109-2111.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_17_2","first-page":"1163","volume":"178","year":"1981","unstructured":"Legendre, A. M., M. Walker, N. Buyukmihci, and R. Stevens. 1981. Canine blastomycosis: a review of 47 clinical cases. J. Am. Vet. Med. Assoc.178:1163-1168.","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-40-5-358"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.7.1710-1717.1994"},{"key":"e_1_3_2_20_2","first-page":"83","volume":"37","year":"1992","unstructured":"Ohara, Y., M. Honma, and Y. Iwasaki. 1992. Sensitivity of the polymerase chain reaction for detecting human T-cell leukemia virus type I sequences in paraffin-embedded tissue. Effect of unbuffered formalin fixation. J. Virol. Methods37:83-88.","journal-title":"J. Virol. Methods"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.748-751.2000"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.1.25-28.1996"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.10.2464-2468.1996"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.1.205-208.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.1.205-208.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T02:15:47Z","timestamp":1646446547000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.41.1.205-208.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1128\/JCM.41.1.205-208.2003"],"URL":"https:\/\/doi.org\/10.1128\/jcm.41.1.205-208.2003","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]},"assertion":[{"value":"2002-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-10-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}