{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:10:35Z","timestamp":1761127835565},"reference-count":37,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            is the causative agent of meningococcal sepsis and meningitis.\n            <jats:italic>Neisseria polysaccharea<\/jats:italic>\n            is a nonpathogenic species.\n            <jats:italic>N. polysaccharea<\/jats:italic>\n            is able to use sucrose to produce amylopectin, a starch-like polysaccharide, which distinguishes it biochemically from the pathogenic species\n            <jats:italic>N. meningitidis<\/jats:italic>\n            . The data presented here indicate that this may be an insufficient criterion to distinguish between these two species. The nonencapsulated\n            <jats:italic>Neisseria<\/jats:italic>\n            strain 93246 expressed a phenotype of amylopectin production similar to that of\n            <jats:italic>N. polysaccharea<\/jats:italic>\n            . However, strain 93246 reacted with\n            <jats:italic>N. meningitidis<\/jats:italic>\n            serotype 4 and serosubtype P1.14 monoclonal antibodies and showed the\n            <jats:italic>N. meningitidis<\/jats:italic>\n            L1(8) lipo-oligosaccharide immunotype. Further analyses were performed on four genetic loci in strain 93246, and the results were compared with 7\n            <jats:italic>N. meningitidis<\/jats:italic>\n            strains, 13\n            <jats:italic>N. polysaccharea<\/jats:italic>\n            strains, and 2\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            strains. Three genetic loci,\n            <jats:italic>opcA<\/jats:italic>\n            ,\n            <jats:italic>siaD<\/jats:italic>\n            , and\n            <jats:italic>lgt-1<\/jats:italic>\n            in strain 93246, were the same as in\n            <jats:italic>N. meningitidis<\/jats:italic>\n            . Particularly, the\n            <jats:italic>siaD<\/jats:italic>\n            gene encoding polysialyltransferase responsible for biosynthesis of\n            <jats:italic>N. meningitidis<\/jats:italic>\n            group B capsule was detected in strain 93246. This\n            <jats:italic>siaD<\/jats:italic>\n            gene was inactivated by a frameshift mutation at the poly(C) tract, which makes strain 93246 identical to other nonencapsulated\n            <jats:italic>N. meningitidis<\/jats:italic>\n            strains. As expected, the\n            <jats:italic>ams<\/jats:italic>\n            gene encoding amylosucrase, responsible for production of amylopectin from sucrose, was detected in strain 93246 and all 13\n            <jats:italic>N. polysaccharea<\/jats:italic>\n            strains but not in\n            <jats:italic>N. meningitidis<\/jats:italic>\n            and\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            strains. These data suggest that strain 93246 is nonencapsulated\n            <jats:italic>N. meningitidis<\/jats:italic>\n            but has the ability to produce extracellular amylopectin from sucrose. The gene for amylopectin production in strain 93246 was likely imported from\n            <jats:italic>N. polysaccharea<\/jats:italic>\n            by horizontal genetic exchange. Therefore, we conclude that genetic analysis is required to complement the traditional phenotypic classification for the nonencapsulated\n            <jats:italic>Neisseria<\/jats:italic>\n            strains.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.1.273-278.2003","type":"journal-article","created":{"date-parts":[[2003,1,7]],"date-time":"2003-01-07T18:03:11Z","timestamp":1041962591000},"page":"273-278","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Nonencapsulated\n            <i>Neisseria meningitidis<\/i>\n            Strain Produces Amylopectin from Sucrose: Altering the Concept for Differentiation between\n            <i>N. meningitidis<\/i>\n            and\n            <i>N. polysaccharea<\/i>"],"prefix":"10.1128","volume":"41","author":[{"given":"Peixuan","family":"Zhu","sequence":"first","affiliation":[{"name":"Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda, Maryland 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond S. W.","family":"Tsang","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao-Ming","family":"Tsai","sequence":"additional","affiliation":[{"name":"Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda, Maryland 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1987.tb02626.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.2.465-466.2001"},{"key":"e_1_3_2_4_2","first-page":"41","volume":"54","year":"1978","unstructured":"Blackwell, C., H. Young, and S. S. Bain. 1978. Isolation of Neisseria meningitidis and Neisseria catarrhalis from the genitourinary tract and anal canal. Br. J. Vener. 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