{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T02:12:02Z","timestamp":1770430322681,"version":"3.49.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2003,10,1]],"date-time":"2003-10-01T00:00:00Z","timestamp":1064966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Sequence analysis of the\n            <jats:italic>penA<\/jats:italic>\n            gene, encoding penicillin-binding protein 2 (PBP2), in 30 penicillin-intermediate (PenI)\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            strains showed altered gene sequences due to the translocation of exogenous DNA blocks derived from commensal neisseriae, which are known to have PBP2 proteins with decreased affinity for the antibiotic. In order to obtain a rapid and reproducible method for predicting the PenI phenotype, a real-time PCR assay was set up with primers and probes designed on the basis of the\n            <jats:italic>penA<\/jats:italic>\n            gene. The A\u2192G mutation at codon 566, in the transpeptidase domain of the\n            <jats:italic>penA<\/jats:italic>\n            gene (which is present in the whole sample of 30 PenI strains and in all the 41 sequences of PenI meningococci isolated worldwide and has been deposited in the sequence databank), was chosen as a marker of\n            <jats:italic>penA<\/jats:italic>\n            translocations. Two hybridization probes were designed to distinguish the wild-type\n            <jats:italic>penA<\/jats:italic>\n            gene in penicillin-susceptible (PenS) meningococci from the mutated\n            <jats:italic>penA<\/jats:italic>\n            gene at codon 566 in PenI strains. Thermal analysis of probe hybridization revealed a melting temperature difference of at least 6\u00b0C between PenI and PenS strains. This real-time PCR protocol characterizes the penicillin phenotype of\n            <jats:italic>N. meningitidis<\/jats:italic>\n            in a few hours without DNA sequencing and is useful for rapid screening of the penicillin-intermediate genotype among meningococcal isolates.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.10.4666-4670.2003","type":"journal-article","created":{"date-parts":[[2003,10,7]],"date-time":"2003-10-07T19:00:26Z","timestamp":1065553226000},"page":"4666-4670","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Prediction of Decreased Susceptibility to Penicillin of\n            <i>Neisseria meningitidis<\/i>\n            Strains by Real-Time PCR"],"prefix":"10.1128","volume":"41","author":[{"given":"Paola","family":"Stefanelli","sequence":"first","affiliation":[{"name":"Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita\u0301, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Carattoli","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita\u0301, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arianna","family":"Neri","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita\u0301, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Fazio","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita\u0301, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Mastrantonio","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita\u0301, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.3.285"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/368170"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.2.333-337.1994"},{"key":"e_1_3_2_5_2","first-page":"779","year":"1983","unstructured":"Dillon, J. R., M. Pauz\u00e9, and K. H. Young. 1983. Spread of penicillinase-producing and transfer plasmids from the gonococcus to Neisseria meningitidis. Lanceti:779-781.","journal-title":"Lancet"},{"key":"e_1_3_2_6_2","first-page":"863","volume":"335","year":"1990","unstructured":"Jones, D. M., and E. M. Sutcliffe. 1990. Meningococci with reduced susceptibility to penicillin. Lancet335:863-864.","journal-title":"Lancet"},{"key":"e_1_3_2_7_2","first-page":"149","volume":"128","year":"2002","unstructured":"Kyaw, M. H., J. C. Bramley, S. Clarke, P. Christie, I. G. Jones, and H. Campbell. 2002. Prevalence of moderate penicillin resistant invasive Neisseria meningitidis infection in Scotland, 1994-9. Epidemiol. Infect.128:149-156.","journal-title":"Epidemiol. Infect."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/346154"},{"key":"e_1_3_2_9_2","unstructured":"National Committee for Clinical Laboratory Standards. 2001. Performance standards for antimicrobial susceptibility testing: 11th information supplement. NCCLS document M100-S11. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(01)00362-4"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9825091"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(01)00289-9"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.4.699"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.22.8988"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5459.1809"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.8.1523"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.10.4666-4670.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.10.4666-4670.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:54:41Z","timestamp":1646445281000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.41.10.4666-4670.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,10]]},"references-count":16,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2003,10]]}},"alternative-id":["10.1128\/JCM.41.10.4666-4670.2003"],"URL":"https:\/\/doi.org\/10.1128\/jcm.41.10.4666-4670.2003","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,10]]},"assertion":[{"value":"2003-03-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-07-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}