{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:36:42Z","timestamp":1790890602241,"version":"4.1.0"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2003,10,1]],"date-time":"2003-10-01T00:00:00Z","timestamp":1064966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A 5\u2032 nuclease PCR assay for detection of the\n            <jats:italic>Yersinia pestis<\/jats:italic>\n            plasminogen activator (\n            <jats:italic>pla<\/jats:italic>\n            ) gene in human respiratory specimens with simulated\n            <jats:italic>Y. pestis<\/jats:italic>\n            infection was developed. An internal positive control was added to the reaction mixture in order to detect the presence of PCR inhibitors that are often found in biological samples. The assay was 100% specific for\n            <jats:italic>Y. pestis<\/jats:italic>\n            . In the absence of inhibitors, a sensitivity of 10\n            <jats:sup>2<\/jats:sup>\n            CFU\/ml of respiratory fluid was obtained. When inhibitors were present, detection of\n            <jats:italic>Y. pestis<\/jats:italic>\n            DNA required a longer sample treatment time and an initial concentration of bacteria of at least 10\n            <jats:sup>4<\/jats:sup>\n            CFU\/ml. The test's total turnaround time was less than 5 h. The assay described here is well suited to the rapid diagnosis of pneumonic plague, the form of plague most likely to result from a bioterrorist attack.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.10.4873-4875.2003","type":"journal-article","created":{"date-parts":[[2003,10,7]],"date-time":"2003-10-07T19:00:26Z","timestamp":1065553226000},"page":"4873-4875","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":73,"title":["Detection of\n            <i>Yersinia pestis<\/i>\n            in Sputum by Real-Time PCR"],"prefix":"10.1128","volume":"41","author":[{"given":"Caroline","family":"Loi\u0308ez","sequence":"first","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Centre Hospitalier Re\u0301gional Universitaire de Lille"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ste\u0301phanie","family":"Herwegh","sequence":"additional","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Centre Hospitalier Re\u0301gional Universitaire de Lille"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fre\u0301de\u0301ric","family":"Wallet","sequence":"additional","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Centre Hospitalier Re\u0301gional Universitaire de Lille"},{"name":"Equipe Mixte Inserm\u2014Universite\u0301 0364, Institut de Biologie de Lille, Lille"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Armand","sequence":"additional","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Centre Hospitalier Re\u0301gional Universitaire de Lille"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"Guinet","sequence":"additional","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence des Yersinia, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene\u0301 J.","family":"Courcol","sequence":"additional","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Centre Hospitalier Re\u0301gional Universitaire de Lille"},{"name":"Equipe Mixte Inserm\u2014Universite\u0301 0364, Institut de Biologie de Lille, Lille"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.3.758-759.1993"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1980-1984.1999"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.8.2284-2288.1998"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.6.1511-1514.1993"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2000.0295"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2000.00384.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1994.tb01622.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/35097083"},{"key":"e_1_3_2_10_2","unstructured":"Pollitzer R. 1954. 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