{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:58:54Z","timestamp":1790074734010,"version":"4.0.1"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2003,11,1]],"date-time":"2003-11-01T00:00:00Z","timestamp":1067644800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            To evaluate the usefulness of a real-time PCR for\n            <jats:italic>Leishmania<\/jats:italic>\n            DNA in the diagnosis and follow-up of patients with human immunodeficiency virus type 1 (HIV-1) and\n            <jats:italic>Leishmania<\/jats:italic>\n            coinfection,\n            <jats:italic>Leishmania<\/jats:italic>\n            DNA levels were measured in whole peripheral blood from 25 HIV-infected patients with clinical features suggestive of visceral leishmaniasis.\n            <jats:italic>Leishmania<\/jats:italic>\n            DNA was detected in 10 of 25 patients with microscopically confirmed visceral leishmaniasis and in none of those without this disease. Following treatment with liposomal amphotericin B, a clinical response was observed in 9 of 10 patients, in association with significantly decreased parasite loads. Seven patients relapsed clinically a median of 110 days after the end of treatment, in association with substantial increases in\n            <jats:italic>Leishmania<\/jats:italic>\n            DNA levels.\n            <jats:italic>Leishmania<\/jats:italic>\n            DNA levels correlated with the clinical course of visceral leishmaniasis, and their measurement at diagnosis and during and after treatment seems to be useful in the clinical management of HIV-infected patients with this disease.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.11.5080-5084.2003","type":"journal-article","created":{"date-parts":[[2003,11,6]],"date-time":"2003-11-06T19:21:05Z","timestamp":1068146465000},"page":"5080-5084","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":93,"title":["Real-Time PCR Assay for Clinical Management of Human Immunodeficiency Virus-Infected Patients with Visceral Leishmaniasis"],"prefix":"10.1128","volume":"41","author":[{"given":"Simona","family":"Bossolasco","sequence":"first","affiliation":[{"name":"Clinic of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Gaiera","sequence":"additional","affiliation":[{"name":"Clinic of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Olchini","sequence":"additional","affiliation":[{"name":"Clinic of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Gulletta","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Tropical Medicine, Spedali Civili, Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Martello","sequence":"additional","affiliation":[{"name":"Clinic of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arabella","family":"Bestetti","sequence":"additional","affiliation":[{"name":"Clinic of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Bossi","sequence":"additional","affiliation":[{"name":"Central Laboratory Analysis, Ospedale Maggiore, IRCCS, Milan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Germagnoli","sequence":"additional","affiliation":[{"name":"Central Laboratory Analysis, San Raffaele Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano","family":"Lazzarin","sequence":"additional","affiliation":[{"name":"Clinic of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Uberti-Foppa","sequence":"additional","affiliation":[{"name":"Clinic of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Cinque","sequence":"additional","affiliation":[{"name":"Clinic of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(96)90538-6"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.109.2.e27"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(02)90075-1"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199606001-00014"},{"key":"e_1_3_1_6_2","first-page":"152","volume":"13","year":"1999","unstructured":"Jimenez-Exposito, M. 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AIDS12:2419-2422.","journal-title":"AIDS"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.236-240.2000"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199803263381301"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.3.746-749.1994"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1198-743x.2001.00262.x"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-200101000-00006"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.1.357-361.2001"},{"key":"e_1_3_1_15_2","first-page":"159","volume":"94","year":"2000","unstructured":"Rosenthal, E., P. Marty, Y. le Fichoux, and J. Cassuto. 2000. Clinical manifestations of visceral leishmaniasis associated with HIV infection: a retrospective study of 91 French cases. Ann. Trop. Med. Parasitol.94:159-162.","journal-title":"Ann. Trop. Med. 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