{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T20:38:14Z","timestamp":1793479094871,"version":"4.5.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2003,2,1]],"date-time":"2003-02-01T00:00:00Z","timestamp":1044057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            There is an increasing demand for diagnostic testing for\n            <jats:italic>Giardia intestinalis (G. lamblia)<\/jats:italic>\n            and\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            , with a priority being placed on obtaining diagnostic results in an efficient and timely manner. Several commercial companies have developed rapid diagnostic tests that are simple to perform and can be completed in less time than traditional methods for detecting\n            <jats:italic>Giardia<\/jats:italic>\n            and\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            . We compared one of these rapid tests, the ImmunoCard STAT! (Meridian Bioscience, Inc.) lateral-flow immunoassay, with the MERIFLUOR direct fluorescent-antibody (DFA) test, the ProSpecT EZ microplate assay for\n            <jats:italic>Giardia<\/jats:italic>\n            and the ProSpecT microplate assay for\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            , and modified Kinyoun's acid-fast stained smears for the detection of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            using 246 specimens. The MERIFLUOR DFA (Meridian Bioscience, Inc.) test detected the largest number of cases (32\n            <jats:italic>Giardia<\/jats:italic>\n            and 37\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            ) infections and was used to calculate the sensitivity and specificity of the other tests. For\n            <jats:italic>Giardia<\/jats:italic>\n            , the sensitivities of the ImmunoCard STAT! and the ProSpecT\n            <jats:italic>Giardia<\/jats:italic>\n            EZ microplate assay (Alexon-Trend, Inc.) were 81 and 91%, respectively. For detection of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            , the sensitivities of the ImmunoCard STAT!, the ProSpecT\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            microplate assay (Alexon-Trend, Inc.), and modified Kinyoun's acid-fast stained smears were 68, 70, and 78%, respectively. Test specificities were equal to or greater than 99%. Specimens with very small numbers of organisms were not detected by the ImmunoCard STAT!, the ProSpecT microplate assay or modified Kinyoun's acid-fast stained smears.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.2.623-626.2003","type":"journal-article","created":{"date-parts":[[2003,2,6]],"date-time":"2003-02-06T20:03:31Z","timestamp":1044561811000},"page":"623-626","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":211,"title":["Evaluation of Three Commercial Assays for Detection of\n            <i>Giardia<\/i>\n            and\n            <i>Cryptosporidium<\/i>\n            Organisms in Fecal Specimens"],"prefix":"10.1128","volume":"41","author":[{"given":"Stephanie P.","family":"Johnston","sequence":"first","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Public Health Service, Department of Health and Human Services"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa M.","family":"Ballard","sequence":"additional","affiliation":[{"name":"the Atlanta Research and Education Foundation, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Beach","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Public Health Service, Department of Health and Human Services"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Causer","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Public Health Service, Department of Health and Human Services"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia P.","family":"Wilkins","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Public Health Service, Department of Health and Human Services"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Ash L. 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