{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:23:50Z","timestamp":1792607030334,"version":"4.5.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2003,2,1]],"date-time":"2003-02-01T00:00:00Z","timestamp":1044057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Cryptococcus neoformans<\/jats:italic>\n            var.\n            <jats:italic>gattii<\/jats:italic>\n            is a causative agent of cryptococcosis and is thought to have a specific ecological association with a number of\n            <jats:italic>Eucalyptus<\/jats:italic>\n            species in Australia. However, the role that the tree plays in the life cycle of the fungus and the nature of the infectious propagule are not well understood. This study set out to examine whether sexual recombination is occurring in a natural population of\n            <jats:italic>C. neoformans<\/jats:italic>\n            var.\n            <jats:italic>gattii<\/jats:italic>\n            and whether the fungus disseminates between colonized trees. Thirty\n            <jats:italic>C. neoformans<\/jats:italic>\n            var.\n            <jats:italic>gattii<\/jats:italic>\n            isolates, consisting of both the \u03b1 and\n            <jats:italic>a<\/jats:italic>\n            mating types, were collected from 13\n            <jats:italic>Eucalyptus camaldulensis<\/jats:italic>\n            trees growing along a riverbank in Renmark, South Australia. The genetic diversity within the population was studied by using amplified fragment length polymorphism fingerprinting, and each isolate was assigned a unique multilocus genotype. Population genetic analyses of the multilocus data found no evidence of genetic exchange between members of the population, indicating a clonal population structure. Canonical variate analysis was then used to study the relationship between isolates from different colonized trees. Isolates from individual trees were strongly correlated, and it appeared that dispersal between trees was not occurring to any appreciable extent. These results suggest that the eucalypt may not be the primary niche for\n            <jats:italic>C. neoformans<\/jats:italic>\n            var.\n            <jats:italic>gattii<\/jats:italic>\n            but that the decaying wood present in hollows on these trees may provide a favorable substrate for extensive clonal propagation of the yeast cells.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.2.703-711.2003","type":"journal-article","created":{"date-parts":[[2003,2,6]],"date-time":"2003-02-06T20:03:31Z","timestamp":1044561811000},"page":"703-711","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":71,"title":["Clonal Reproduction and Limited Dispersal in an Environmental Population of\n            <i>Cryptococcus neoformans<\/i>\n            var.\n            <i>gattii<\/i>\n            Isolates from Australia"],"prefix":"10.1128","volume":"41","author":[{"given":"C. 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