{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T19:23:37Z","timestamp":1792265017708,"version":"4.4.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2003,3,1]],"date-time":"2003-03-01T00:00:00Z","timestamp":1046476800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Several\n            <jats:italic>Bartonella<\/jats:italic>\n            species have now been implicated as human pathogens. The recovery of these fastidious organisms in the clinical microbiology laboratory remains difficult, and current methods are still relatively insensitive. Thus, the bartonellae are good candidates for detection by PCR. We have developed a PCR assay which uses a single primer pair targeting the riboflavin synthase gene (\n            <jats:italic>ribC<\/jats:italic>\n            ) and detected six\n            <jats:italic>Bartonella<\/jats:italic>\n            species that have been implicated in human disease,\n            <jats:italic>B. henselae<\/jats:italic>\n            ,\n            <jats:italic>B. quintana<\/jats:italic>\n            ,\n            <jats:italic>B. bacilliformis<\/jats:italic>\n            ,\n            <jats:italic>B. clarridgeiae<\/jats:italic>\n            ,\n            <jats:italic>B. elizabethae<\/jats:italic>\n            , and\n            <jats:italic>B. vinsonii<\/jats:italic>\n            subsp.\n            <jats:italic>berkhoffii.<\/jats:italic>\n            Species identification is achieved simply by restriction enzyme digestion of the amplicon. This PCR assay appears to be specific for the\n            <jats:italic>Bartonella<\/jats:italic>\n            genus because it failed to amplify DNA from several other bacterial species.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.3.1069-1072.2003","type":"journal-article","created":{"date-parts":[[2003,3,6]],"date-time":"2003-03-06T19:07:00Z","timestamp":1046977620000},"page":"1069-1072","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["Detection and Identification of\n            <i>Bartonella<\/i>\n            Species Pathogenic for Humans by PCR Amplification Targeting the Riboflavin Synthase Gene (\n            <i>ribC<\/i>\n            )"],"prefix":"10.1128","volume":"41","author":[{"given":"G.","family":"Johnson","sequence":"first","affiliation":[{"name":"Division of Microbiology, Hospital for Sick Children, University of Toronto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ayers","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Hospital for Sick Children, University of Toronto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. C. C.","family":"McClure","sequence":"additional","affiliation":[{"name":"Cabbagetown Pet Clinic, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. E.","family":"Richardson","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Hospital for Sick Children, University of Toronto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Tellier","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Hospital for Sick Children, University of Toronto"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.2.203"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/24094"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1438-4221(00)80081-8"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.10.3159-3166.1999"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2000.0289"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.4.872-881.1993"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.6.1327-1331.1997"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)68237-5"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10684.x"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.1717-1722.2000"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.9.3274-3279.2000"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4034-4038.1999"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.7.1813-1818.1997"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.5.1536-1547.1999"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1899-1905.1999"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-3-1015"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4045-4047.1999"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.7.1797-1803.1995"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.6.1573-1579.1995"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.4.1698-1700.2000"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.173.2.697-703.1991"},{"key":"e_1_3_1_23_2","unstructured":"Welch D. F. and L. N. Slater. 1999. Bartonella and Afipia p. 638-646. In P. R. Murray E. J. Barron M. A. Pfaller F. C. Tenover and R. H. Yolken (ed.) Manual of clinical microbiology 7th ed. American Society for Microbiology Washington D.C."},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2598-2601.1999"},{"key":"e_1_3_1_25_2","first-page":"165","year":"2002","unstructured":"Zeaiter, Z., P.-E. Fournier, H. Ogata, and D. Raoult. 2002. Phylogenetic classification of Bartonella species by comparing groEL sequences. Int. J. Syst. Evol. Microbiol. 52:165-171.","journal-title":"Int. J. Syst. 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