{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:06:53Z","timestamp":1790852813207,"version":"4.1.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The virulence genotype profile and presence of a pathogenicity island(s) (PAI) were studied in 18 strains of F165-positive\n            <jats:italic>Escherichia coli<\/jats:italic>\n            originally isolated from diseased calves or piglets. On the basis of their adhesion phenotypes and genotypes, these extraintestinal pathogenic strains were classified into three groups. The F165 fimbrial complex consists of at least two serologically and genetically distinct fimbriae: F165\n            <jats:sub>1<\/jats:sub>\n            and F165\n            <jats:sub>2<\/jats:sub>\n            . F165\n            <jats:sub>1<\/jats:sub>\n            is encoded by the\n            <jats:italic>foo<\/jats:italic>\n            operon (\n            <jats:italic>pap<\/jats:italic>\n            -like), and F165\n            <jats:sub>2<\/jats:sub>\n            is encoded by\n            <jats:italic>fot<\/jats:italic>\n            (\n            <jats:italic>sfa<\/jats:italic>\n            related). Strains in group 1 were\n            <jats:italic>foo<\/jats:italic>\n            and\n            <jats:italic>fot<\/jats:italic>\n            positive, strains in group 2 were\n            <jats:italic>foo<\/jats:italic>\n            and\n            <jats:italic>afa<\/jats:italic>\n            positive, and strains in group 3 were\n            <jats:italic>foo<\/jats:italic>\n            positive only. The strains were tested for the presence of virulence genes found mainly in extraintestinal pathogenic\n            <jats:italic>E. coli<\/jats:italic>\n            (ExPEC) strains. Although all the strains were positive for the\n            <jats:italic>papA<\/jats:italic>\n            variant encoding F11 fimbriae\n            <jats:italic>incD<\/jats:italic>\n            ,\n            <jats:italic>traT<\/jats:italic>\n            , and\n            <jats:italic>papC<\/jats:italic>\n            , the prevalence of virulence genes commonly found in PAIs associated with ExPEC strains was highly variable, with strains of group 2 harboring most of the virulence genes tested.\n            <jats:italic>papG<\/jats:italic>\n            allele III was detected in all strains in group 1 and in one strain in group 3. All other strains were negative for the known alleles encoding PapG adhesins. The association of virulence genes with tRNA genes was characterized in these strains by using pulsed-field gel electrophoresis and DNA hybridization. The insertion site of the\n            <jats:italic>foo<\/jats:italic>\n            operon was found at the\n            <jats:italic>pheU<\/jats:italic>\n            tRNA locus in 16 of the 18 strains and at the\n            <jats:italic>selC<\/jats:italic>\n            tRNA locus in the other 2 strains. Furthermore, 8 of the 18 strains harbored a high-pathogenicity island which was inserted in either the\n            <jats:italic>asnT<\/jats:italic>\n            or the\n            <jats:italic>asnV<\/jats:italic>\n            \/\n            <jats:italic>U<\/jats:italic>\n            tRNA locus. These results suggest the presence of one or more PAIs in septicemic strains from animals and the association of the\n            <jats:italic>foo<\/jats:italic>\n            operon with at least one of these islands. F165-positive strains share certain virulence traits with ExPEC, and most of them are pathogenic in piglets, as tested in experimental infections.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.4.1375-1385.2003","type":"journal-article","created":{"date-parts":[[2003,4,7]],"date-time":"2003-04-07T20:34:54Z","timestamp":1049747694000},"page":"1375-1385","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Presence and Characterization of Extraintestinal Pathogenic\n            <i>Escherichia coli<\/i>\n            Virulence Genes in F165-Positive\n            <i>E. coli<\/i>\n            Strains Isolated from Diseased Calves and Pigs"],"prefix":"10.1128","volume":"41","author":[{"given":"Hojabr","family":"Dezfulian","sequence":"first","affiliation":[{"name":"Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculte\u0301 de Me\u0301decine Ve\u0301te\u0301rinaire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Batisson","sequence":"additional","affiliation":[{"name":"Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculte\u0301 de Me\u0301decine Ve\u0301te\u0301rinaire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Fairbrother","sequence":"additional","affiliation":[{"name":"Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculte\u0301 de Me\u0301decine Ve\u0301te\u0301rinaire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C. K.","family":"Lau","sequence":"additional","affiliation":[{"name":"Environmental Biotechnology Sector, Biotechnology Research Institute, National Research Council of Canada, Montreal, Que\u0301bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atef","family":"Nassar","sequence":"additional","affiliation":[{"name":"Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculte\u0301 de Me\u0301decine Ve\u0301te\u0301rinaire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Szatmari","sequence":"additional","affiliation":[{"name":"De\u0301partement de Microbiologie et Immunologie, Faculte\u0301 de Me\u0301decine, Universite\u0301 de Montre\u0301al, Saint-Hyacinthe"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301e","family":"Harel","sequence":"additional","affiliation":[{"name":"Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculte\u0301 de Me\u0301decine Ve\u0301te\u0301rinaire"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb08973.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03078.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1220"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13004.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/514217"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.35.2.654-659.1982"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","unstructured":"Birren B. 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