{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T03:58:51Z","timestamp":1777348731743,"version":"3.51.4"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Maternal prenatal screening for group B streptococci (GBS) followed by offering of intrapartum chemoprophylaxis to carriers is one of the strategies used to reduce the incidence of neonatal early-onset GBS infections. Culturing of vaginal and anorectal swab specimens in selective broth is the screening procedure recommended by the Centers for Disease Control and Prevention. This technique is sensitive; it does not, however, allow either evaluation of the degree of colonization or detection of cocolonization with several GBS clones. We have examined the carriage rate and population dynamics of GBS in a group of Danish women during pregnancy and 1 year after delivery using a new detection method. In the present paper we describe a mixed blood agar medium (MB agar) that identifies GBS in the primary cultures by detection of a double hemolysis pattern consisting of characteristic, large zones of partial hemolysis (\u201cCAMP zones\u201d) and of narrow zones of complete hemolysis. The MB agar was at least as sensitive as culturing in selective broth for detection of GBS in vaginal and anorectal swab specimens, and GBS strains could be identified directly on the primary plate due to the CAMP zones without the need for subculturing. The carriage rate of GBS in a group of Danish women was found to be more than 30%, a figure considerably higher than the rate that was reported previously.<\/jats:p>","DOI":"10.1128\/jcm.41.4.1399-1403.2003","type":"journal-article","created":{"date-parts":[[2003,4,7]],"date-time":"2003-04-07T20:34:54Z","timestamp":1049747694000},"page":"1399-1403","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Method for Quantitative Detection and Presumptive Identification of Group B Streptococci on Primary Plating"],"prefix":"10.1128","volume":"41","author":[{"given":"S\u00f8ren Mose","family":"Hansen","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus C, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uffe B. Skov","family":"S\u00f8rensen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus C, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/137.5.524"},{"key":"e_1_3_2_3_2","unstructured":"Armstrong D. 1995. Listeisteria monocytogenes p. 1880-1885. In G. L. Mandell J. E. Bennett and R. Dolin (ed.) Mandell Douglas and Bennett's principles and practice of infectious diseases. Churchill Livingstone New York N.Y."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1139\/m80-091"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.23.3.838-844.1979"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/338258"},{"key":"e_1_3_2_7_2","first-page":"1","volume":"45","year":"1996","unstructured":"Centers for Disease Control and Prevention. 1996. Prevention of perinatal group B streptococcal disease: a public health perspective. Morb. Mortal. Wkly. Rep.45:1-24.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_8_2","first-page":"793","volume":"49","year":"2000","unstructured":"Centers for Disease Control and Prevention. 2000. Early-onset group B streptococcal disease\u2014United States, 1998-1999. Morb. Mortal. Wkly. Rep.49:793-796.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1944.26"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.1.2.171-174.1975"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/84.2.216"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.9.6.665-672.1979"},{"key":"e_1_3_2_13_2","first-page":"351","volume":"17","year":"1988","unstructured":"Fehrenbach, F. J., D. Jurgens, J. Ruhlmann, B. Sterzik, and M. \u00d6zel. 1988. Role of CAMP-factor (protein B) for virulence. Zentbl. Bakteriol. Parasitenkd. Infektkrankh. Hyg. Abt. 1 Orig. Suppl.17:351-357.","journal-title":"Zentbl. Bakteriol. Parasitenkd. Infektkrankh. Hyg. Abt. 1 Orig. Suppl."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2001.109936"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.7.2.232-233.1978"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4725-4731.1999"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1139\/w00-078"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1995.tb07407.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1996.0028"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.3.720"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/46.3.324"},{"key":"e_1_3_2_22_2","unstructured":"Kilian M. 1998. Streptococcus and Lactobacillus p. 633-667. In A. Balows and B. I. Duerden (ed.) Topley and Wilson's microbiology and microbial infections 9th ed. Arnold London United Kingdom."},{"key":"e_1_3_2_23_2","first-page":"1036","volume":"99","year":"2002","unstructured":"Orafu, C., P. Gill, C. Nelson, B. Hecht, and M. Hopkins. 2002. Perianal versus anorectal specimens: is there a difference in group B streptococcal detection? Obstet. Gynecol.99:1036-1039.","journal-title":"Obstet. Gynecol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.12.2.135-137.1980"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00198458"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.24.2.296-297.1986"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(96)81053-9"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)81275-4"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200001063420103"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007203430310"},{"key":"e_1_3_2_31_2","first-page":"190","volume":"249","year":"1981","unstructured":"Skalka, B., and J. Smola. 1981. Lethal effect of CAMP-factor and uberis-factor: a new finding about diffusible exosubstances of Streptococcus agalactiae and Streptococcus uberis. Zentbl. Bakteriol. Parasitenkd. Infektkrankh. Hyg. Abt. 1 Orig. Reihe A249:190-194.","journal-title":"Zentbl. Bakteriol. Parasitenkd. Infektkrankh. Hyg. Abt. 1 Orig. Reihe A"},{"key":"e_1_3_2_32_2","first-page":"817","volume":"131","year":"1985","unstructured":"Sterzik, B., and F. J. Fehrenbach. 1985. Reaction components influencing CAMP factor induced lysis. J. Gen. Microbiol.131:817-820.","journal-title":"J. Gen. Microbiol."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.4.1399-1403.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.4.1399-1403.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T02:17:56Z","timestamp":1646446676000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.41.4.1399-1403.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1128\/JCM.41.4.1399-1403.2003"],"URL":"https:\/\/doi.org\/10.1128\/jcm.41.4.1399-1403.2003","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4]]},"assertion":[{"value":"2002-07-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-11-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}