{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T18:56:55Z","timestamp":1789930615176,"version":"4.0.1"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A number of methicillin-resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA) isolates were recovered over a period of several weeks from blood samples and from the heart valve of a patient who underwent extensive vancomycin chemotherapy for persistent\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>aureus<\/jats:italic>\n                    bacteremia. Consecutive isolates showed gradually decreasing growth rates during in vitro cultivation and increasing vancomycin MICs, from an MIC of 1 \u03bcg\/ml for the initial isolate to an MIC of 8 \u03bcg\/ml for the final MRSA isolates, which also became tolerant to vancomycin. Major changes were observed in the oxacillin resistance phenotype of several of the isolates\u2014apparently related to in vivo exposure to imipenem, which was also used during a period of chemotherapy. Both the gradually increasing vancomycin MICs and the changes in oxacillin resistance could be reproduced by appropriate exposure of the initial MRSA isolate to antibiotics in vitro. All isolates had the same pulsed-field gel electrophoresis pattern,\n                    <jats:italic>spaA<\/jats:italic>\n                    type, and multilocus sequence type (MLST), which was identified as a single-locus variant of ST5, the MLST characteristic of previously characterized MRSA isolates with reduced susceptibility to vancomycin in the United States and Japan.\n                  <\/jats:p>","DOI":"10.1128\/jcm.41.4.1687-1693.2003","type":"journal-article","created":{"date-parts":[[2003,4,7]],"date-time":"2003-04-07T16:34:54Z","timestamp":1049733294000},"page":"1687-1693","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":119,"title":["Evolution of a Vancomycin-Intermediate\n                    <i>Staphylococcus aureus<\/i>\n                    Strain In Vivo: Multiple Changes in the Antibiotic Resistance Phenotypes of a Single Lineage of Methicillin-Resistant\n                    <i>S<\/i>\n                    .\n                    <i>aureus<\/i>\n                    under the Impact of Antibiotics Administered for Chemotherapy"],"prefix":"10.1128","volume":"41","author":[{"given":"K.","family":"Sieradzki","sequence":"first","affiliation":[{"name":"The Rockefeller University, New York, New York 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Leski","sequence":"additional","affiliation":[{"name":"The Rockefeller University, New York, New York 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Dick","sequence":"additional","affiliation":[{"name":"John Hopkins Medical Institutions, Baltimore, Maryland 21287"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Borio","sequence":"additional","affiliation":[{"name":"John Hopkins Medical Institutions, Baltimore, Maryland 21287"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Tomasz","sequence":"additional","affiliation":[{"name":"The Rockefeller University, New York, New York 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Aires-de-Sousa M. H. de Lencastre I. S. Sanches K. Kikuchi K. Totsuka and A. Tomasz. 2000. Similarity of antibiotic resistance patterns and molecular typing properties of methicillin-resistant Staphylococcus aureus isolates widely spread in hospitals in New York city and in a hospital in Tokyo Japan. Microb. Drug Resist. 6: 253-258.","DOI":"10.1089\/mdr.2000.6.253"},{"key":"e_1_3_2_3_2","unstructured":"Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl (ed.). 1995. Current protocols in molecular biology p. 241-242. John Wiley & Sons Inc. New York N.Y."},{"key":"e_1_3_2_4_2","first-page":"813","volume":"46","year":"1997","unstructured":"Centers for Disease Control and Prevention. 1997. Update: Staphylococcus aureus with reduced susceptibility to vancomycin\u2014United States, 1998. Morb. Mortal. Wkly. Rep.46:813-815.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2000.6.189"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.29.5.797"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.161272898"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.9.1824"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1008-1015.2000"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/40.1.135"},{"key":"e_1_3_2_11_2","unstructured":"NCCLS. 2000. Performance standards for antimicrobial disk susceptibility test. NCCLS Wayne Pa."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.7.2155-2161.2002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(02)00227-X"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)79166-2"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2000.6.245"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.11.3556-3563.1999"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.24.7566-7570.1999"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199902183400704"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.8.2557-2566.1997"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.27.18942"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.1.124"},{"key":"e_1_3_2_22_2","first-page":"1297","volume":"132","year":"1986","unstructured":"Tuomanen, E., R. Cozens, W. Tosch, O. Zak, and A. Tomasz. 1986. The rate of killing of Escherichia coli by beta-lactam antibiotics is strictly proportional to the rate of bacterial growth. J. Gen. Microbiol.132:1297-1304.","journal-title":"J. Gen. Microbiol."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.4.1687-1693.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.4.1687-1693.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T21:18:05Z","timestamp":1646428685000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.41.4.1687-1693.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1128\/JCM.41.4.1687-1693.2003"],"URL":"https:\/\/doi.org\/10.1128\/jcm.41.4.1687-1693.2003","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4]]},"assertion":[{"value":"2002-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-01-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}