{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:12:27Z","timestamp":1759104747029},"reference-count":6,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A method based on solid-phase cytometry for the detection and enumeration of single cells of\n            <jats:italic>Cryptococcus neoformans<\/jats:italic>\n            in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.4.1736-1737.2003","type":"journal-article","created":{"date-parts":[[2003,4,7]],"date-time":"2003-04-07T20:34:54Z","timestamp":1049747694000},"page":"1736-1737","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Detection of Single Cells of\n            <i>Cryptococcus neoformans<\/i>\n            in Clinical Samples by Solid-Phase Cytometry"],"prefix":"10.1128","volume":"41","author":[{"given":"T. G. M.","family":"Bauters","sequence":"first","affiliation":[{"name":"Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Swinne","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Stove","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University Hospital, Ghent"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. J.","family":"Nelis","sequence":"additional","affiliation":[{"name":"Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.39.4.379.381"},{"key":"e_1_3_2_3_2","first-page":"461","volume":"9","year":"2002","unstructured":"Bialek, R., M. Weiss, K. Bekure-Nemariam, L. K. Najvar, M. B. Alberdi, J. R. Graybill, and U. Reischl. 2002. Detection of Cryptococcus neoformans DNA in tissue samples by nested and real-time PCR assays. Clin. Diagn. Lab. Immunol.9:461-469.","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"Casadevall A. and J. R. Perfect. 1998. Diagnosis and laboratory techniques p. 381-405. In A. Casadevall and J. R. Perfect (ed.) Cryptococcus neoformans . 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