{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:06:56Z","timestamp":1767164816200,"version":"build-2238731810"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The complex relation between the genotype of\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    and its association with clinical outcome is not well understood. Studies in the West have showed that strains expressing certain virulence factors (\n                    <jats:italic>vacAs1<\/jats:italic>\n                    ,\n                    <jats:italic>vacAm1<\/jats:italic>\n                    , and\n                    <jats:italic>cagA<\/jats:italic>\n                    ) are associated with duodenal ulcer disease. However, the\n                    <jats:italic>H. pylori<\/jats:italic>\n                    genotype is known to vary with geographic region. In the present study, we compared several virulence markers (\n                    <jats:italic>cagA<\/jats:italic>\n                    ,\n                    <jats:italic>vacA<\/jats:italic>\n                    , and\n                    <jats:italic>iceA<\/jats:italic>\n                    ) and neutral markers (IS\n                    <jats:italic>605<\/jats:italic>\n                    , IS\n                    <jats:italic>606<\/jats:italic>\n                    , and IS\n                    <jats:italic>608<\/jats:italic>\n                    ) in\n                    <jats:italic>H. pylori<\/jats:italic>\n                    strains isolated from 65 adult patients with peptic ulcer (PU) and 50 patients with nonulcer dyspepsia (NUD). PCR tests indicated that\n                    <jats:italic>cagA<\/jats:italic>\n                    is present in 75% of the strains from patients with PU compared to 55% in patients with NUD, and 80% of the isolates from patients with PU carried potentially toxigenic\n                    <jats:italic>vacAs1<\/jats:italic>\n                    alleles of the vacuolating cytotoxin gene (\n                    <jats:italic>vacA<\/jats:italic>\n                    ) compared to 60% in isolates from patients with NUD. However, no significant difference in any other virulence marker was observed in isolates from both groups. Phylogenetic analysis of the\n                    <jats:italic>vacA<\/jats:italic>\n                    middle region and the 5\u2032 end of the\n                    <jats:italic>cagA<\/jats:italic>\n                    gene indicates that Bangladeshi isolates are more closely related to\n                    <jats:italic>H. pylori<\/jats:italic>\n                    isolates from India and are different from isolates from East Asia.\n                  <\/jats:p>","DOI":"10.1128\/jcm.41.5.2008-2014.2003","type":"journal-article","created":{"date-parts":[[2003,5,6]],"date-time":"2003-05-06T19:28:47Z","timestamp":1052249327000},"page":"2008-2014","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["DNA-Level Characterization of\n                    <i>Helicobacter pylori<\/i>\n                    Strains from Patients with Overt Disease and with Benign Infections in Bangladesh"],"prefix":"10.1128","volume":"41","author":[{"given":"Motiur","family":"Rahman","sequence":"first","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh"},{"name":"Departments of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asish K.","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"National Institute of Cholera and Enteric Disease, Calcutta, India"},{"name":"Departments of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shamsun","family":"Nahar","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simanti","family":"Datta","sequence":"additional","affiliation":[{"name":"National Institute of Cholera and Enteric Disease, Calcutta, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milan","family":"Mashhud Ahmad","sequence":"additional","affiliation":[{"name":"and Dhaka Medical College Hospital, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Safique","family":"Sarker","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ibna M.","family":"Masud","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Engstrand","sequence":"additional","affiliation":[{"name":"SMI, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. John","family":"Albert","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Balakrish","family":"Nair","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas E.","family":"Berg","sequence":"additional","affiliation":[{"name":"Departments of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01382.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1046\/j.1365-2958.1998.00770.x","volume":"28","year":"1998","unstructured":"Akopyants, N. S., S. W. Clifton, D. 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