{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T23:12:12Z","timestamp":1784934732636,"version":"3.55.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2003,6,1]],"date-time":"2003-06-01T00:00:00Z","timestamp":1054425600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genetic relationship of 81 ampicillin-resistant and 21 ampicillin-susceptible\n            <jats:italic>Enterococcus faecium<\/jats:italic>\n            isolates from clinical infections and rectal screening in hospitalized patients in Norway was studied by pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP). PFGE showed 55 different banding patterns, and 65 of the isolates could be grouped into one large group. With AFLP, 46 patterns were discerned, and 74 isolates clustered in one group. In general, the isolates had a higher degree of similarity than with PFGE. The\n            <jats:italic>purK<\/jats:italic>\n            gene, which is one of the targets of the\n            <jats:italic>E. faecium<\/jats:italic>\n            multilocus sequence typing scheme, was sequenced. Eleven different\n            <jats:italic>purK<\/jats:italic>\n            alleles could be discerned, with the majority of isolates (\n            <jats:italic>n<\/jats:italic>\n            = 80) harboring allele 1. With only two exceptions, all strains carrying\n            <jats:italic>purK-1<\/jats:italic>\n            clustered in the same PFGE and AFLP groups, indicating a good correlation between PFGE type, AFLP type, and\n            <jats:italic>purK<\/jats:italic>\n            allele. Genetic polymorphism of a 571-bp PCR fragment of the C-terminal domain of the penicillin-binding protein 5 gene (\n            <jats:italic>pbp5<\/jats:italic>\n            ) was determined, and sequence differences were associated with the level of ampicillin resistance. This study indicates that the majority of ampicillin-resistant\n            <jats:italic>E. faecium<\/jats:italic>\n            strains in Norway belong to a distinct genetic lineage of closely related genotypes. Rectal and clinical isolates were generally indistinguishable, and differences in clonal distribution and allele polymorphism were found mainly between ampicillin-resistant and -susceptible isolates.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.6.2330-2336.2003","type":"journal-article","created":{"date-parts":[[2003,6,5]],"date-time":"2003-06-05T19:14:27Z","timestamp":1054840467000},"page":"2330-2336","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Molecular Characterization of Ampicillin-Resistant\n            <i>Enterococcus faecium<\/i>\n            Isolates from Hospitalized Patients in Norway"],"prefix":"10.1128","volume":"41","author":[{"given":"Roland","family":"Jureen","sequence":"first","affiliation":[{"name":"Institute of Medicine, University of Bergen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janetta","family":"Top","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute for Public Health and the Environment, Bilthoven, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stein Christian","family":"Mohn","sequence":"additional","affiliation":[{"name":"Institute of Medicine, University of Bergen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stig","family":"Harthug","sequence":"additional","affiliation":[{"name":"Department of Medicine, Haukeland University Hospital, Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Langeland","sequence":"additional","affiliation":[{"name":"Institute of Medicine, University of Bergen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob J. L.","family":"Willems","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute for Public Health and the Environment, Bilthoven, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1802","volume":"357","year":"2001","unstructured":"Baldassarri, L., L. Bertuccini, M. G. Ammendolia, G. Gherardi, and R. Creti. 2001. Variant esp gene in vancomycin-sensitive Enterococcus faecium. 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