{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:52:01Z","timestamp":1792515121672,"version":"4.5.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2003,6,1]],"date-time":"2003-06-01T00:00:00Z","timestamp":1054425600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The demand for molecular diagnostic tests in medical microbiology has highlighted the need for efficient methods of DNA extraction. In addition, it is preferable for these methods to be automated. An example of such a requirement is for the confirmation of meningococcal disease where rapid, sensitive, and specific procedures are required for public health management purposes. Previous studies have shown that whole blood is the preferred method for the isolation of bacterial DNA in meningococcal disease, and in this study, we compare five commercially available kits for the extraction of bacterial genomic DNA from whole-blood samples. These include kits in a 96-well binding plate, 96-well filter plate, and metallic bead formats. The method for all five kits is described, and the sensitivity, specificity, ease of automation, and overall efficiency are determined.<\/jats:p>","DOI":"10.1128\/jcm.41.6.2440-2443.2003","type":"journal-article","created":{"date-parts":[[2003,6,5]],"date-time":"2003-06-05T19:14:27Z","timestamp":1054840467000},"page":"2440-2443","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Comparison of Commercial DNA Extraction Kits for Extraction of Bacterial Genomic DNA from Whole-Blood Samples"],"prefix":"10.1128","volume":"41","author":[{"given":"K.","family":"Smith","sequence":"first","affiliation":[{"name":"Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Diggle","sequence":"additional","affiliation":[{"name":"Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. C.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital"},{"name":"Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.13.2623"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb12862.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268896007261"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.10142"},{"key":"e_1_3_2_6_2","first-page":"230","volume":"58","year":"2001","unstructured":"Clarke, S. C., M. A. Diggle, and G. F. Edwards. 2001. Automated non-culture-based sequence typing of meningococci from body fluids. Br. J. Biomed. Sci.58:230-234.","journal-title":"Br. J. Biomed. Sci."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.9.3066-3071.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1553-1558.2001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4518-4519.2001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2000.tb01473.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","unstructured":"Harris D. M. Engelstein R. Parry J. Smith M. Mabuchi and J. L. Millipore. 2002. High-speed plasmid isolation using 96-well size-exclusion filter plates. BioTechniques 32 : 626-628 630-631.","DOI":"10.2144\/02323pf02"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(02)00018-0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1661-1669.1999"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/mp.54.2.86"},{"key":"e_1_3_2_15_2","first-page":"416","volume":"8","year":"2002","unstructured":"Rengarajan, K., S. M. Cristol, M. Mehta, and J. M. Nickerson. 2002. Quantifying DNA concentrations using fluorometry: a comparison of fluorophores. Mol. Vis.8:416-421.","journal-title":"Mol. 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