{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:37:07Z","timestamp":1762864627087},"reference-count":24,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2003,6,1]],"date-time":"2003-06-01T00:00:00Z","timestamp":1054425600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            To determine the prevalence and genotype of an extended-spectrum beta-lactamase and new chromosomal AmpC beta-lactamases among clinical isolates of\n            <jats:italic>Enterobacter<\/jats:italic>\n            species, we performed antibiotic susceptibility testing, pI determination, induction tests, transconjugation, enterobacterial repetitive consensus (ERIC) PCR, sequencing, and phylogenetic analysis. Among the 51 clinical isolates collected from a university hospital in Korea, 6 isolates have been shown to produce SHV-12 and inducible AmpC beta-lactamases. These also included three isolates producing TEM-1b and one strain carrying TEM-1b and CMY-type beta-lactamases with a pI of 8.0. The results from ERIC PCR revealed that six isolates were genetically unrelated, suggesting that dissemination of SHV-12 was responsible for the spread of resistance to extended-spectrum beta-lactams in Korea. Six genes of inducible AmpC beta-lactamases that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized. A 1,165-bp DNA fragment containing the\n            <jats:italic>ampC<\/jats:italic>\n            genes was sequenced and found to have an open reading frame coding for a 381-amino-acid beta-lactamase. The nucleotide sequence of four\n            <jats:italic>ampC<\/jats:italic>\n            genes (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EcloK992004.1<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EcloK995120.1<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EcloK99230<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EareK9911729<\/jats:sub>\n            ) shared considerable homology with that of AmpC-type class C beta-lactamase genes of gram-negative bacteria, especially that of the chromosomal\n            <jats:italic>ampC<\/jats:italic>\n            gene (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EcloMHN1<\/jats:sub>\n            ) of\n            <jats:italic>Enterobacter cloacae<\/jats:italic>\n            MHN1 (99.9, 99.7, 99.6, and 99.6% identity, respectively). The sequences of two\n            <jats:italic>ampC<\/jats:italic>\n            genes (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EcloK9973<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EcloK9914325<\/jats:sub>\n            ) showed close similarity to the chromosomal\n            <jats:italic>ampC<\/jats:italic>\n            gene (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EcloQ908R<\/jats:sub>\n            ) of\n            <jats:italic>E. cloacae<\/jats:italic>\n            Q908R (99.7% identity). The results from phylogenetic analysis suggested that six\n            <jats:italic>ampC<\/jats:italic>\n            genes could originate from\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EcloMHN1<\/jats:sub>\n            or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EcloQ908R<\/jats:sub>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.41.6.2477-2482.2003","type":"journal-article","created":{"date-parts":[[2003,6,5]],"date-time":"2003-06-05T19:14:27Z","timestamp":1054840467000},"page":"2477-2482","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Dissemination of SHV-12 and Characterization of New AmpC-Type Beta-Lactamase Genes among Clinical Isolates of\n            <i>Enterobacter<\/i>\n            Species in Korea"],"prefix":"10.1128","volume":"41","author":[{"given":"Sang Hee","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Genetic Engineering, Youngdong University, Chungbuk 370-701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Biotechnology, Graduate School of Biological Sciences, Seoul National University, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Heum","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering, Youngdong University, Chungbuk 370-701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Jun","family":"An","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering, Youngdong University, Chungbuk 370-701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Wook","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering, Youngdong University, Chungbuk 370-701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeun Chang","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering, Youngdong University, Chungbuk 370-701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ha Il","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering, Youngdong University, Chungbuk 370-701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eui Suk","family":"Sohn","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering, Youngdong University, Chungbuk 370-701"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seok Hoon","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Kosin University College of Medicine, Pusan"},{"name":"Research Institute of Bacterial Resistance, Yonsei University College of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kye Joon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Biotechnology, Graduate School of Biological Sciences, Seoul National University, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.5.1190-1198.2002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.8.1926"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.3.563"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-47-3-227"},{"key":"e_1_3_2_7_2","unstructured":"Farmer J. J. III. 1995. Enterobacteriaceae : introduction and identification p. 438-449. In P. R. Murray E. J. Baron M. A. Pfaller F. C. Tenover and R. H. Yolken (ed.) Manual of clinical microbiology 3rd ed. American Society for Microbiology Washington D.C."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.45.100191.000345"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90540-R"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.7.1759"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.4.867"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.5.1446-1449.1998"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765x.2002.01088.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.2.269"},{"key":"e_1_3_2_15_2","first-page":"157","volume":"200","year":"2001","unstructured":"Lee, S. H., J. Y. Kim, S. H. Shin, S. K. Lee, M. M. Choi, I. Y. Lee, Y. B. Kim, J. Y. Cho, W. Jin, and K. J. Lee. 2001. Restriction fragment length dimorphism-PCR method for the detection of extended-spectrum beta-lactamases unrelated to TEM- and SHV-types. FEMS Microbiol. Lett.200:157-161.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2001.00202.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.557"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13131.x"},{"key":"e_1_3_2_19_2","unstructured":"National Committee for Clinical Laboratory Standards. 1999. Performance standards for antimicrobial susceptibility testing 9th ed. Informational supplement. M100-S9. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.3.596"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3158-3162.2000"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.3.647"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.2.220"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.6.2477-2482.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.6.2477-2482.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T02:26:01Z","timestamp":1646447161000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.41.6.2477-2482.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,6]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2003,6]]}},"alternative-id":["10.1128\/JCM.41.6.2477-2482.2003"],"URL":"https:\/\/doi.org\/10.1128\/jcm.41.6.2477-2482.2003","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,6]]},"assertion":[{"value":"2002-09-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-02-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}