{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T17:23:45Z","timestamp":1697304225118},"reference-count":19,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2003,6,1]],"date-time":"2003-06-01T00:00:00Z","timestamp":1054425600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Human herpesvirus 8 (HHV-8) can be classified into distinct subtypes on the basis of sequence polymorphisms in several open reading frames (ORFs). We analyzed the subtypes of HHV-8 in 59 human immunodeficiency virus-infected Japanese patients by using polymorphisms in ORF26 and found that over two-thirds of the HHV-8 isolates fell into major subtype A. We also found that single nucleotide polymorphisms (SNPs) at nucleotide positions 1032 (C-to-A substitution) and 1055 (G-to-T substitution) in HHV-8 ORF26 were correlated with increased susceptibility to Kaposi's sarcoma, compared to the results obtained with HHV-8 with wild-type nucleotides at these positions (\n            <jats:italic>P<\/jats:italic>\n            = 0.0106). This observation suggests that molecular heterogeneity of the HHV-8 genome affects the biological properties of HHV-8, resulting in different clinical phenotypes of HHV-8 infection. Since sensitive PCR of ORF26 allowed us to analyze the SNPs by using peripheral blood from HHV-8-infected patients, the ORF26 SNPs will be a potent tool for investigating the pathogenesis of HHV-8 infection.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.6.2492-2497.2003","type":"journal-article","created":{"date-parts":[[2003,6,5]],"date-time":"2003-06-05T19:14:27Z","timestamp":1054840467000},"page":"2492-2497","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Molecular Analysis of Human Herpesvirus 8 by Using Single Nucleotide Polymorphisms in Open Reading Frame 26"],"prefix":"10.1128","volume":"41","author":[{"given":"Tokiomi","family":"Endo","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Advanced Clinical Research Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyuki","family":"Miura","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Advanced Clinical Research Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiko","family":"Koibuchi","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Applied Immunology, The Institute of Medical Science, The University of Tokyo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitomi","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Advanced Clinical Research Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Advanced Clinical Research Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Odawara","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Applied Immunology, The Institute of Medical Science, The University of Tokyo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mieko","family":"Goto","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Advanced Clinical Research Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Ajisawa","sequence":"additional","affiliation":[{"name":"Tokyo Metropolitan Komagome Hospital, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aikichi","family":"Iwamoto","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Advanced Clinical Research Center"},{"name":"Department of Infectious Diseases and Applied Immunology, The Institute of Medical Science, The University of Tokyo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Applied Immunology, The Institute of Medical Science, The University of Tokyo"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90001-L"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/314471"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.52.1.453"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7997879"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.5.4156-4170.1999"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199902)57:2<159::AID-JMV12>3.0.CO;2-A"},{"key":"e_1_3_2_8_2","first-page":"233","volume":"355","year":"1996","unstructured":"Gao, S. J., L. Kingsley, D. R. Hoover, T. J. Spira, C. R. Rinaldo, Saah, J. Phair, R. Detels, P. Parry, Y. Chang, and P. Moor. 1996. Seroconversion to antibodies against Kaposi's sarcoma-associated herpesvirus-related latent nuclear antigens before the development of Kaposi's sarcoma. N. Engl. J. Med.355:233-241.","journal-title":"N. Engl. J. Med."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0896-925"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(79)90731-9"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.8.3478-3485.2000"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199911)59:3<346::AID-JMV15>3.0.CO;2-4"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0896-918"},{"key":"e_1_3_2_14_2","first-page":"4226","volume":"6","year":"2000","unstructured":"Ma, H. J., N. N. Sjak-Shie, R. A. Vescio, M. Kaminsky, A. Mikail, M. Pold, K. Parker, M. Beksac, D. Belson, T. J. Moss, C. H. Wu, J. Zhou, L. Zhang, G. Chen, J. W. Said, and J. R. Berenson. 2000. Human herpesvirus 8 open reading frame 26 and open reading frame 65 sequences from multiple myeloma patients: a shared pattern not found in Kaposi's sarcoma or primary effusion lymphoma. Clin. Cancer Res.6:4226-4233.","journal-title":"Clin. Cancer Res."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-82-3-499"},{"key":"e_1_3_2_16_2","first-page":"79","volume":"23","year":"1998","unstructured":"Nicholas, J., J. C. Zong, D. J. Alcendor, D. M. Cifo, L. J. Poole, R. T. Sarisky, C. J. Chiou, X. Zhang, X. Wan, H. G. Guo, M. S. Reitz, and G. S. Hayward. 1998. Novel organizational features, captured cellular genes and strain variability within the genome of KSHV\/HHV8. J. Natl. Cancer Inst. Monogr.23:79-88.","journal-title":"J. Natl. Cancer Inst. Monogr."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.8.6646-6660.1999"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468-1293.2000.00034.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)91619-9"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.6.2492-2497.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.6.2492-2497.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T02:21:40Z","timestamp":1646446900000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.41.6.2492-2497.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,6]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2003,6]]}},"alternative-id":["10.1128\/JCM.41.6.2492-2497.2003"],"URL":"https:\/\/doi.org\/10.1128\/jcm.41.6.2492-2497.2003","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,6]]},"assertion":[{"value":"2002-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-02-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}