{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T15:20:00Z","timestamp":1776180000005,"version":"3.50.1"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2003,6,1]],"date-time":"2003-06-01T00:00:00Z","timestamp":1054425600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Primarily saprophytic in nature, fungi of the genus\n            <jats:italic>Acremonium<\/jats:italic>\n            are a well-documented cause of mycetoma and other focal diseases. More recently, a number of\n            <jats:italic>Acremonium<\/jats:italic>\n            spp. have been implicated in invasive infections in the setting of severe immunosuppression. During the course of routine microbiological studies involving a case of fatal mycosis in a nonmyeloablative hematopoietic stem cell transplant patient, we identified a greater-than-expected variation among strains previously identified as\n            <jats:italic>Acremonium strictum<\/jats:italic>\n            by clinical microbiologists. Using DNA sequence analysis of the ribosomal DNA intergenic transcribed spacer (ITS) regions and the D1-D2 variable domain of the 28S ribosomal DNA gene (28S), the case isolate and four other clinical isolates phenotypically identified as\n            <jats:italic>A. strictum<\/jats:italic>\n            were found to have &lt;99% homology to the\n            <jats:italic>A. strictum<\/jats:italic>\n            type strain, CBS 346.70, at the ITS and 28S loci, while a sixth isolate phenotypically identified only as\n            <jats:italic>Acremonium<\/jats:italic>\n            sp\n            <jats:italic>.<\/jats:italic>\n            had &gt;99% homology to the type strain at both loci. These results suggest that five out of the six clinical isolates belong to species other than\n            <jats:italic>A. strictum<\/jats:italic>\n            or that the\n            <jats:italic>A. strictum<\/jats:italic>\n            taxon is genetically diverse. Based upon these sequence data, the clinical isolates were placed into three genogroups.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.6.2623-2628.2003","type":"journal-article","created":{"date-parts":[[2003,6,5]],"date-time":"2003-06-05T19:14:27Z","timestamp":1054840467000},"page":"2623-2628","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Genetic Diversity among Clinical Isolates of\n            <i>Acremonium strictum<\/i>\n            Determined during an Investigation of a Fatal Mycosis"],"prefix":"10.1128","volume":"41","author":[{"given":"Thomas J.","family":"Novicki","sequence":"first","affiliation":[{"name":"Departments of Laboratory Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"LaFe","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynda","family":"Bui","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uyen","family":"Bui","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Geise","sequence":"additional","affiliation":[{"name":"Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kieren","family":"Marr","sequence":"additional","affiliation":[{"name":"Medicine"},{"name":"Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brad T.","family":"Cookson","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine"},{"name":"Microbiology, University of Washington"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.4042-4051.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.6.2302-2310.2000"},{"key":"e_1_3_2_4_2","unstructured":"Domsch K. 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