{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:53:30Z","timestamp":1790844810870,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A screening method for methicillin-resistant\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            (MRSA) by using selective broth and real-time PCR (broth-PCR) was developed and evaluated. The samples (\n            <jats:italic>n<\/jats:italic>\n            = 304) were cultured in the broth overnight, followed by\n            <jats:italic>nuc<\/jats:italic>\n            gene detection by real-time PCR.\n            <jats:italic>nuc<\/jats:italic>\n            -negative samples were further checked for the presence of\n            <jats:italic>nuc<\/jats:italic>\n            amplification inhibitors by a PCR internal inhibitor assay.\n            <jats:italic>nuc<\/jats:italic>\n            -positive samples and\n            <jats:italic>nuc<\/jats:italic>\n            -negative samples with PCR inhibitors were cultured onto plates and processed further. The diagnostic values for this MRSA screening method were 93.3% sensitivity, 89.6% specificity, 31.8% positive predictive value, and 99.6% negative predictive value. The application of the broth-PCR method will be able to report most of the negative samples (258 of 289 [89.3%]) on the next morning and can save as much as 84.9% (258 of 304) of the labor and cost spent on processing the\n            <jats:italic>nuc<\/jats:italic>\n            -negative specimens on plates. In the study, all the samples were processed in parallel by the broth enrichment method and the plating method for comparison. To identify MRSA, the isolated oxacillin-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            strains were tested by a duplex real-time PCR targeting the\n            <jats:italic>mecA<\/jats:italic>\n            gene and the\n            <jats:italic>nuc<\/jats:italic>\n            gene. A collection of MRSA, methicillin-susceptible\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            , methicillin-resistant\n            <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n            , and methicillin-susceptible\n            <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n            strains and a panel of standard strains of 11 bacterial species other than\n            <jats:italic>S. aureus<\/jats:italic>\n            were also tested by this method, which was proved to be a valuable tool for MRSA identification in a routine microbiological laboratory.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.7.2894-2899.2003","type":"journal-article","created":{"date-parts":[[2003,7,3]],"date-time":"2003-07-03T19:05:48Z","timestamp":1057259148000},"page":"2894-2899","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":155,"title":["Rapid Screening and Identification of Methicillin-Resistant\n            <i>Staphylococcus aureus<\/i>\n            from Clinical Samples by Selective-Broth and Real-Time PCR Assay"],"prefix":"10.1128","volume":"41","author":[{"given":"Hong","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Clinical Bacteriology, Huddinge University Hospital, SE-141 86 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Go\u0308ran","family":"Hedin","sequence":"additional","affiliation":[{"name":"Department of Clinical Bacteriology, Huddinge University Hospital, SE-141 86 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/502092"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.7.1654-1660.1992"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.282.18.1745"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.781"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.122108599"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.10.3727-3732.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2392-2397.2002"},{"key":"e_1_3_2_9_2","first-page":"1","volume":"91","year":"1999","unstructured":"Jarlov, J. 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