{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:43:48Z","timestamp":1767851028734,"version":"3.49.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Comparative sequence analysis was performed upon\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            and its closest relatives,\n            <jats:italic>B. cereus<\/jats:italic>\n            and\n            <jats:italic>B. thuringiensis<\/jats:italic>\n            . Portions of\n            <jats:italic>rpoB<\/jats:italic>\n            DNA from 10 strains of\n            <jats:italic>B. anthracis<\/jats:italic>\n            , 16 of\n            <jats:italic>B. cereus<\/jats:italic>\n            , 10 of\n            <jats:italic>B. thuringiensis<\/jats:italic>\n            , 1 of\n            <jats:italic>B. mycoides<\/jats:italic>\n            , and 1 of\n            <jats:italic>B. megaterium<\/jats:italic>\n            were amplified and sequenced. The determined\n            <jats:italic>rpoB<\/jats:italic>\n            sequences (318 bp) of the 10\n            <jats:italic>B. anthracis<\/jats:italic>\n            strains, including five Korean isolates, were identical to those of Ames, Florida, Kruger B, and Western NA strains. Strains of the \u201c\n            <jats:italic>B. cereus<\/jats:italic>\n            group\u201d were separated into two subgroups, in which the\n            <jats:italic>B. anthracis<\/jats:italic>\n            strains formed a separate clade in the phylogenetic tree. However,\n            <jats:italic>B. cereus<\/jats:italic>\n            and\n            <jats:italic>B. thuringiensis<\/jats:italic>\n            could not be differentiated. Sequence analysis confirmed the five Korean isolates as\n            <jats:italic>B. anthracis<\/jats:italic>\n            . Based on the\n            <jats:italic>rpoB<\/jats:italic>\n            sequences determined in the present study, multiplex PCR generating either\n            <jats:italic>B. anthracis<\/jats:italic>\n            -specific amplicons (359 and 208 bp) or\n            <jats:italic>cap<\/jats:italic>\n            DNA (291 bp) in a virulence plasmid could be used for the rapid differential detection and identification of virulent\n            <jats:italic>B. anthracis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.41.7.2908-2914.2003","type":"journal-article","created":{"date-parts":[[2003,7,3]],"date-time":"2003-07-03T19:05:48Z","timestamp":1057259148000},"page":"2908-2914","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Identification of\n            <i>Bacillus anthracis<\/i>\n            by\n            <i>rpoB<\/i>\n            Sequence Analysis and Multiplex PCR"],"prefix":"10.1128","volume":"41","author":[{"given":"Kwan Soo","family":"Ko","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital, Seoul 110-799"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong-Man","family":"Kim","sequence":"additional","affiliation":[{"name":"Animal Disease Research Department"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong-Wan","family":"Kim","sequence":"additional","affiliation":[{"name":"Animal Disease Research Department"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byeong Yeal","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, National Veterinary Research and Quarantine Service, Kyunggi Province, 430-824"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wonyong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Chung-Ang University College of Medicine and Institute of Medical Research, Seoul 156-756"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ik Jung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Dongguk University, Gyungju, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoon-Hoh","family":"Kook","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital, Seoul 110-799"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-41-3-343"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.4.1623-1626.1995"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.3.1298-1303.1999"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199909093411107"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.7.2425-2430.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.4.1333-1338.2002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11324.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.22.6951"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.7.1847-1850.1995"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.4.1615-1622.2000"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.6.2627-2630.2000"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.17.2236"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.3.1224"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1714-1720.1999"},{"key":"e_1_3_2_16_2","first-page":"21","volume":"207","year":"2001","unstructured":"Kim, W., Y. 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