{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:34:04Z","timestamp":1791120844660,"version":"4.1.0"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The nucleotide sequences (534 to 546 bp) of the\n            <jats:italic>groEL<\/jats:italic>\n            gene, which encodes the 60-kDa heat shock protein GroEL, from 15 rickettsial strains were determined and compared. In the phylogenetic tree created by the unweighted pair group method with arithmetic averages and the neighbor-joining method, rickettsial strains could be distinguished from\n            <jats:italic>Ehrlichia<\/jats:italic>\n            strains. Five spotted fever group strains, four typhus group strains, and six scrub typhus group (STG) strains were differentiated as distinct entities. Unlike\n            <jats:italic>gltA<\/jats:italic>\n            and\n            <jats:italic>ompA<\/jats:italic>\n            gene analyses, differentiation between members of the genus\n            <jats:italic>Rickettsia<\/jats:italic>\n            and the STG rickettsiae by\n            <jats:italic>groEL<\/jats:italic>\n            gene analysis was possible. In comparison with 16S rRNA gene analysis, the\n            <jats:italic>groEL<\/jats:italic>\n            gene has a higher degree of divergence among the rickettsiae. We therefore successfully developed rapid differentiation methods, PCR-restriction fragment length polymorphism analysis and a species-specific PCR, based on the\n            <jats:italic>groEL<\/jats:italic>\n            gene sequences. Four Korean isolates were identified by these methods and\n            <jats:italic>groEL<\/jats:italic>\n            gene analysis. The results suggest that the\n            <jats:italic>groEL<\/jats:italic>\n            gene is useful for the identification and characterization of rickettsiae\n            <jats:italic>.<\/jats:italic>\n          <\/jats:p>","DOI":"10.1128\/jcm.41.7.2952-2960.2003","type":"journal-article","created":{"date-parts":[[2003,7,3]],"date-time":"2003-07-03T19:05:48Z","timestamp":1057259148000},"page":"2952-2960","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Differentiation of Rickettsiae by\n            <i>groEL<\/i>\n            Gene Analysis"],"prefix":"10.1128","volume":"41","author":[{"given":"Jung-Hee","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyo-Soon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Jong","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong-Eun","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, College of Medicine, Konkuk University, Chungju, Chungchongbuk-Do 380-701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-Moon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, College of Medicine, Konkuk University, Chungju, Chungchongbuk-Do 380-701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo-Kyoung","family":"Shim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Institute of Health, Seoul 122-701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Yeoun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Institute of Health, Seoul 122-701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon-Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do 200-702"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bum-Joon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Seoul National University College of Medicine, Seoul 110-799, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon-Hoh","family":"Kook","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Seoul National University College of Medicine, Seoul 110-799, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Hee","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"359","volume":"25","year":"1990","unstructured":"Back, L. 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