{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:43:05Z","timestamp":1775151785317,"version":"3.50.1"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A healthy vaginal ecosystem has been shown to be protective against the acquisition of human immunodeficiency virus and gonorrhea, and women who are colonized with H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            -producing lactobacilli are more likely to maintain a normal vaginal flora than women with lactobacilli that do not produce H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            . The purpose of this study was to formulate a testing medium that better supports the growth and detection of H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            by a broader range of lactobacilli than a published, widely used agar formulation (TMB). The new medium (TMB-Plus) consists of brucella agar base, 3,3\u2032,5,5\u2032-tetramethylbenzidine, horseradish peroxidase, starch, vitamin K, hemin, magnesium sulfate, manganese sulfate, and horse serum. To validate the new formula, 256 vaginal isolates and ATCC strains were inoculated onto TMB-Plus and, for comparison, onto TMB. Growth was enhanced for 69% of the isolates on TMB-Plus, and 48% had enhanced color production. The percentage of H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            -positive isolates increased from 71% on TMB to 79% on TMB-Plus. Formulations using Rogosa or MRS agar base in combination with peroxidase and a chromogen did not support the growth of all of the strains of\n            <jats:italic>Lactobacillus<\/jats:italic>\n            , and fewer H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            -producing strains were detected on these formulations than on TMB-Plus. This new medium better supports the growth of a wider range of\n            <jats:italic>Lactobacillus<\/jats:italic>\n            strains isolated from the vagina and enhances the color production of H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            -producing strains.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.7.3260-3264.2003","type":"journal-article","created":{"date-parts":[[2003,7,3]],"date-time":"2003-07-03T19:05:48Z","timestamp":1057259148000},"page":"3260-3264","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Optimization of Media for Detection of Hydrogen Peroxide Production by\n            <i>Lactobacillus<\/i>\n            Species"],"prefix":"10.1128","volume":"41","author":[{"given":"L. K.","family":"Rabe","sequence":"first","affiliation":[{"name":"Magee-Women's Research Institute"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. L.","family":"Hillier","sequence":"additional","affiliation":[{"name":"Magee-Women's Research Institute"},{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, The University of Pittsburgh, Pittsburgh, Pennsylvania 15213"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/315109"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.12.6558-6564.1999"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.2.251-256.1989"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.3.729-733.1999"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1990.tb01523.x"},{"key":"e_1_3_2_7_2","first-page":"377","volume":"10","year":"1987","unstructured":"Giorgi, A., S. Torriani, F. Dellaglio, G. Bo, E. Stola, and L. Bernuzzi. 1987. Identification of vaginal lactobacilli from asymptomatic women. Microbiologica10:377-384.","journal-title":"Microbiologica"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.5.1058"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-30-1-53"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/315127"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/165.6.1142"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(80)90340-3"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.62.1.132-133.1951"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108024"},{"key":"e_1_3_2_15_2","first-page":"223","volume":"9","year":"1960","unstructured":"Sharpe, M. E. 1960. Selective media for the isolation and enumeration of lactobacilli. Lab. Practice9:223-227.","journal-title":"Lab. 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