{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T16:26:07Z","timestamp":1772641567834,"version":"3.50.1"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Vaginal swab specimens may be preferable to cervical swab or urine specimens for the detection of\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            and\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            because of the ease of specimen collection and transport. The purpose of this study was to evaluate whether vaginal swab specimens are equivalent to cervical swab specimens for the detection of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            and\n            <jats:italic>C. trachomatis<\/jats:italic>\n            by the Becton Dickinson strand displacement amplification assay (SDA) with the BDProbeTec ET instrument and then to evaluate the use of the amplification control in a clinical research setting. In the first phase, vaginal and cervical swab specimens were obtained from 455 symptomatic women aged 18 to 40 attending primary health care and sexually transmitted disease clinics. Thirty-nine specimens (8.6%) had true-positive results for\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            and 37 specimens (8.1%) had true-positive results for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            . The sensitivity of SDA was superior to that of culture for the detection of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            with vaginal swab specimens and equivalent to that of the Roche PCR for the detection of\n            <jats:italic>C. trachomatis<\/jats:italic>\n            with cervical swab specimens. In the second phase of the study, 1,411 consecutively collected vaginal swab specimens were evaluated, with 357 (25.3%) specimens giving indeterminate readings on the basis of the result for the amplification control. The prevalences of sexually transmitted pathogens in vaginal swab specimens with and without use of the amplification control were 6.0 and 5.8%, respectively, for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and 3.1 and 3.0%, respectively, for\n            <jats:italic>N. gonorrhoeae.<\/jats:italic>\n            Although, vaginal swab specimens were equivalent to cervical swab specimens for the detection of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            and\n            <jats:italic>C. trachomatis<\/jats:italic>\n            by SDA with respect to sensitivity, one in four vaginal swab specimens yielded an indeterminate result when the amplification control was used. The amplification control has limited value for use with vaginal swab specimens.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.8.3592-3596.2003","type":"journal-article","created":{"date-parts":[[2003,8,6]],"date-time":"2003-08-06T20:46:30Z","timestamp":1060202790000},"page":"3592-3596","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Detection of\n            <i>Chlamydia trachomatis<\/i>\n            and\n            <i>Neisseria gonorrhoeae<\/i>\n            by Strand Displacement Amplification and Relevance of the Amplification Control for Use with Vaginal Swab Specimens"],"prefix":"10.1128","volume":"41","author":[{"given":"Lisa A.","family":"Cosentino","sequence":"first","affiliation":[{"name":"Magee-Womens Research Institute"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel V.","family":"Landers","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute"},{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon L.","family":"Hillier","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute"},{"name":"Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.1.160"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.6.1536-1540.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.10.2628-2633.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.2129-2132.1997"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.12.3549-3551.1998"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/132.2.204"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/45.6.777"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.313.7066.1186"},{"key":"e_1_3_2_10_2","first-page":"S19","volume":"21","year":"1994","unstructured":"Quinn, T. C. 1994. Recent advances in diagnosis of sexually transmitted diseases. Sex. Transm. Dis.21:S19-S27.","journal-title":"Sex. Transm. 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