{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:33:48Z","timestamp":1787016828730,"version":"build-2736575974"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,8]]},"abstract":"ABSTRACT<\/jats:title>\n \n The human metapneumovirus (hMPV) is a new member of the\n Paramyxoviridae<\/jats:italic>\n family associated with acute respiratory tract infections in humans. The objective of this study was to compare the sensitivity of real-time RT-PCR assays performed in a LightCycler instrument and designed to amplify the viral nucleoprotein (N), matrix (M), fusion (F), phosphoprotein (P), and polymerase (L) genes. In a first evaluation of 20 viral cultures with characteristics compatible with hMPV cytopathic effect, the PCR positivity rates were 100, 90, 75, 60, and 55% using primers for the N, L, M, P, and F genes. In a second evaluation of 10 nasopharyngeal aspirates from children with bronchiolitis and found to be positive for the hMPV N gene, the PCR positivity rates for the L, M, P, and F genes were 90, 60, 30, and 80%, respectively. The analytic sensitivity of the real-time RT-PCR assay for the hMPV N gene was 100 copies using a transcribed viral plasmid. In conclusion, real-time PCR assays aimed at amplifying the N and L genes which are coding for two internal viral proteins appear particularly suitable for hMPV diagnostic.\n <\/jats:p>","DOI":"10.1128\/jcm.41.8.3631-3635.2003","type":"journal-article","created":{"date-parts":[[2003,8,6]],"date-time":"2003-08-06T16:46:30Z","timestamp":1060188390000},"page":"3631-3635","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":112,"title":["Comparative Evaluation of Real-Time PCR Assays for Detection of the Human Metapneumovirus"],"prefix":"10.1128","volume":"41","author":[{"given":"Ste\u0301phanie","family":"Co\u0302te\u0301","sequence":"first","affiliation":[{"name":"Infectious Diseases Research Center, Centre Hospitalier Universitaire de Que\u0301bec (CHUQ-CHUL)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yacine","family":"Abed","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Center, Centre Hospitalier Universitaire de Que\u0301bec (CHUQ-CHUL)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Boivin","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Center, Centre Hospitalier Universitaire de Que\u0301bec (CHUQ-CHUL)"},{"name":"Department of Medical Biology, Laval University, Que\u0301bec City, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.9.3135-3139.2001"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/344319"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.817-820.2002"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11391-2"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.100-105.2003"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/315085"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.2001.1044"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.11.3149-3154.1998"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0809.020238"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/340518"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.10.2990-2995.1998"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0809.020084"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.1355"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/89098"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.12.4418-4422.2002"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90007-C"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.8.3631-3635.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.8.3631-3635.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T21:31:54Z","timestamp":1646429514000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.41.8.3631-3635.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8]]},"references-count":16,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["10.1128\/JCM.41.8.3631-3635.2003"],"URL":"https:\/\/doi.org\/10.1128\/jcm.41.8.3631-3635.2003","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8]]},"assertion":[{"value":"2003-01-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-05-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}