{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T17:56:05Z","timestamp":1772214965462,"version":"3.50.1"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Paracoccidioidomycosis (PCM) is an important systemic fungal disease, particularly among individuals living and working in rural areas of endemicity in Latin America, who, without antifungal therapy, may develop fatal acute or chronic infection. For such patients, the detection of antibody responses by immunodiffusion is of limited value due to false-negative results. In contrast, the detection of\n            <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>\n            gp43 circulating antigen may represent a more practical approach to the rapid diagnosis of the disease. Accordingly, an inhibition enzyme-linked immunosorbent assay (inh-ELISA) was developed for the detection of a 43-kDa\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            -specific epitope incorporating a species-specific murine monoclonal antibody. With sera from patients with acute and chronic forms of the disease (\n            <jats:italic>n<\/jats:italic>\n            = 81), the overall sensitivity of the test was found to be 95.1%, while specificity was found to be 97.5% compared to that with normal human sera from blood donors (\n            <jats:italic>n<\/jats:italic>\n            = 93) and sera from patients with other chronic fungal infections (histoplasmosis [\n            <jats:italic>n<\/jats:italic>\n            = 33] and cryptococcosis [\n            <jats:italic>n<\/jats:italic>\n            = 20]). The inh-ELISA detected circulating antigen in 100% of patients with the acute form of PCM and in 95.31 and 100% of patients with the chronic multifocal and unifocal forms of PCM according to the patient's clinical presentation. Cerebrospinal fluid from 14 patients with neuroparacoccidioidomycosis and 13 samples of bronchoalveolar lavage fluid from patients with pulmonary unifocal PCM were also tested for gp43 detection, with the test showing 100% sensitivity and specificity. This novel, highly specific inh-ELISA represents a significant addition to the existing tests for the diagnosis of PCM.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.8.3675-3680.2003","type":"journal-article","created":{"date-parts":[[2003,8,6]],"date-time":"2003-08-06T20:46:30Z","timestamp":1060202790000},"page":"3675-3680","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":82,"title":["Detection of Circulating gp43 Antigen in Serum, Cerebrospinal Fluid, and Bronchoalveolar Lavage Fluid of Patients with Paracoccidioidomycosis"],"prefix":"10.1128","volume":"41","author":[{"given":"Silvia Helena","family":"Marques da Silva","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnaldo Lopes","family":"Colombo","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Federal University of Sa\u0303o Paulo, Sa\u0303o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Heloisa Souza Lima","family":"Blotta","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Medical School, State University of Campinas, Campinas, Sa\u0303o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose\u0301 Daniel","family":"Lopes","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fla\u0301vio","family":"Queiroz-Telles","sequence":"additional","affiliation":[{"name":"Department of Community Health, Medical School, Federal University of Parana\u0301, Curitiba, Parana\u0301, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoilo","family":"Pires de Camargo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.3.671-676.1993"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"Camargo Z. 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