{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T19:33:35Z","timestamp":1789932815674,"version":"4.0.1"},"reference-count":11,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The potency of smallpox vaccines produced in the 1970s was tested by titration onto chorioallantoic membranes of fertilized hen eggs (CAM assay). The potency specification commonly approved for these vaccines was a titer above 10\n            <jats:sup>8<\/jats:sup>\n            pock-forming units per milliliter. We developed and validated a cell culture titration assay to have a more reliable potency test. The cell titration assay and the CAM assay were tested in parallel on 34 first-generation smallpox vaccine lots. These allowed us to demonstrate that a correlation does exist between the two titration techniques and to determine a new in-house specification for the cell titration method. This in vitro potency assay will allow us to test first-generation smallpox vaccines produced on the skin of living animals but will also give a hint of the potency specification that should be assigned for new generations of cell-derived smallpox vaccines.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.8.3687-3689.2003","type":"journal-article","created":{"date-parts":[[2003,8,6]],"date-time":"2003-08-06T20:46:30Z","timestamp":1060202790000},"page":"3687-3689","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["New Generation of Cell Culture Assay for Smallpox Vaccine Potency"],"prefix":"10.1128","volume":"41","author":[{"given":"Isabelle","family":"Leparc-Goffart","sequence":"first","affiliation":[{"name":"Agence Franc\u0327aise de Se\u0301curite\u0301 Sanitaire des Produits de Sante\u0301, 69007 Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Poirier","sequence":"additional","affiliation":[{"name":"Agence Franc\u0327aise de Se\u0301curite\u0301 Sanitaire des Produits de Sante\u0301, 69007 Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie","family":"El Zaouk","sequence":"additional","affiliation":[{"name":"Agence Franc\u0327aise de Se\u0301curite\u0301 Sanitaire des Produits de Sante\u0301, 34740 Vendargues"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-He\u0301le\u0300ne","family":"Tissier","sequence":"additional","affiliation":[{"name":"Agence Franc\u0327aise de Se\u0301curite\u0301 Sanitaire des Produits de Sante\u0301, 93285 Saint-Denis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Agence Franc\u0327aise de Se\u0301curite\u0301 Sanitaire des Produits de Sante\u0301, 69007 Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"31","volume":"100","year":"1999","unstructured":"Arita, I. 1999. Standardization of smallpox vaccines and the eradication programme \u2014 a W.H.O. perspective. Dev. Biol. Stand.100:31-37.","journal-title":"Standardization of smallpox vaccines and the eradication programme \u2014 a W.H.O. perspective. Dev. Biol. Stand."},{"key":"e_1_3_2_3_2","unstructured":"European Pharmacopoeia. 1982. Freeze-dried smallpox vaccine (dermal). European Pharmacopoeia monograph 164. European Pharmacopoeia Strasbourg France."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/4.5.916"},{"key":"e_1_3_2_5_2","unstructured":"Fenner F. D. A. Henderson I. Arita Z. Jezek and I. D. Ladnyi. 1988. Smallpox and its eradication. World Health Organization Geneva Switzerland."},{"key":"e_1_3_2_6_2","unstructured":"Fenner F. 1996. Poxviruses p. 2673-2702. In B. N. Fields D. M. Knipe P. M. Howley et al. (ed.) Field's virology 3rd ed. Lippincott-Raven Publishers Philadelphia Pa."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(92)90353-L"},{"key":"e_1_3_2_8_2","unstructured":"Henderson D. A. and B. Moss. 1999. Smallpox and vaccinia p. 74-97. In S. A. Plotkin and W. A. Orenstein (ed.) Vaccines 3rd ed. W. B. Saunders Company Philadelphia Pa."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(88)90240-1"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.2807\/esm.06.11.00385-en"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-1157(76)90019-6"},{"key":"e_1_3_2_12_2","unstructured":"World Health Organization. 1965. Requirements for smallpox vaccine. W.H.O. Technical Report Series 323. World Health Organization Geneva Switzerland."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.8.3687-3689.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.8.3687-3689.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T02:28:05Z","timestamp":1646447285000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.41.8.3687-3689.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8]]},"references-count":11,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["10.1128\/JCM.41.8.3687-3689.2003"],"URL":"https:\/\/doi.org\/10.1128\/jcm.41.8.3687-3689.2003","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8]]},"assertion":[{"value":"2003-02-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-05-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}