{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:58:09Z","timestamp":1791284289953,"version":"4.1.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A real-time PCR assay was developed for the detection of\n            <jats:italic>Ehrlichia chaffeensis<\/jats:italic>\n            . The assay is species specific and provides quantitative results in the range 10 to 10\n            <jats:sup>10<\/jats:sup>\n            gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for\n            <jats:italic>E. chaffeensis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.41.8.3870-3872.2003","type":"journal-article","created":{"date-parts":[[2003,8,6]],"date-time":"2003-08-06T20:46:30Z","timestamp":1060202790000},"page":"3870-3872","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Quantitative Real-Time PCR Assay for Detection of\n            <i>Ehrlichia chaffeensis<\/i>"],"prefix":"10.1128","volume":"41","author":[{"given":"Amanda D.","family":"Loftis","sequence":"first","affiliation":[{"name":"Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert F.","family":"Massung","sequence":"additional","affiliation":[{"name":"Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Levin","sequence":"additional","affiliation":[{"name":"Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1993.49.239"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.4.775-780.1992"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199907153410303"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.12.2741-2745.1991"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/163.3.564"},{"key":"e_1_3_2_7_2","first-page":"2145","year":"2001","unstructured":"Dumler, J. S., A. F. Barbet, C. P. Bekker, G. A. Dasch, G. H. Palmer, S. C. Ray, Y. Rikihisa, and F. R. Rurangirwa. 2001. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and \u2032HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int. J. Syst. Evol. Microbiol. 51:2145-2165.","journal-title":"Int. J. Syst. E"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/32.3.368"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.3.1419"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.2307\/3284284"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/104454902320308960"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0505.990504"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.10.2496-2502.1997"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.5.1447-1453.1999"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.11.5012-5017.1988"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-2-250"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.4.944-947.1997"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.8.3870-3872.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.8.3870-3872.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T02:31:18Z","timestamp":1646447478000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.41.8.3870-3872.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8]]},"references-count":16,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["10.1128\/JCM.41.8.3870-3872.2003"],"URL":"https:\/\/doi.org\/10.1128\/jcm.41.8.3870-3872.2003","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8]]},"assertion":[{"value":"2003-02-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-05-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}