{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T10:41:22Z","timestamp":1774780882630,"version":"3.50.1"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An aerobic gram-negative bacterium was isolated from the blood and sputum of an 84-year-old, chair-bound nursing home resident with acute bacteremic pneumonia. Although the phenotypic characteristics suggested that the bacterium could be\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            , the Vitek 1 system (GNI+), which can successfully identify 99% of\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            strains, showed that the bacterium was \u201cunidentified.\u201d Immunoglobulin G against the lipopolysaccharide (LPS) of\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            , as detected by an LPS-based enzyme-linked immunosorbent assay with 95% sensitivity, was negative in both the acute-phase and convalescent-phase sera. Sequencing of the\n            <jats:italic>groEL<\/jats:italic>\n            gene showed that the isolate was\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            . Proper identification of the bacterium in this study is crucial, since there would be a radical difference in the duration of antimicrobial therapy.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.8.3973-3977.2003","type":"journal-article","created":{"date-parts":[[2003,8,6]],"date-time":"2003-08-06T20:46:30Z","timestamp":1060202790000},"page":"3973-3977","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Seronegative Bacteremic Melioidosis Caused by\n            <i>Burkholderia pseudomallei<\/i>\n            with Ambiguous Biochemical Profile: Clinical Importance of Accurate Identification by 16S rRNA Gene and\n            <i>groEL<\/i>\n            Gene Sequencing"],"prefix":"10.1128","volume":"41","author":[{"given":"Patrick C. Y.","family":"Woo","sequence":"first","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanna K. P.","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gibson K. S.","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ami M. Y.","family":"Fung","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio H. Y.","family":"Ngan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wai-ting","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwok-yung","family":"Yuen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"},{"name":"HKU-Pasteur Research Centre, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"146","volume":"31","year":"2000","unstructured":"Anuntagool, N., P. Aramsri, T. Panichakul, V. R. Wuthiekanun, R. Kinoshita, N. J. White, and S. 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