{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T14:17:16Z","timestamp":1774793836968,"version":"3.50.1"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Due to temporal changes in the epidemiology of gonorrhea, a precise characterization of\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            is essential. In the present study genetic heterogeneity in the\n            <jats:italic>porB<\/jats:italic>\n            genes of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            was examined, and serovar determination was compared to\n            <jats:italic>porB<\/jats:italic>\n            gene sequencing. Among 108\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolates, phylogenetic analysis of the entire\n            <jats:italic>porB<\/jats:italic>\n            alleles (924 to 993 bp) identified 87 unique sequences. By analyzing only the four to six most heterogeneous\n            <jats:italic>porB<\/jats:italic>\n            gene regions (174 to 363 bp), 86 out of these 87 genetic variants were identified. Consequently, analysis of shorter highly variable regions of the\n            <jats:italic>porB<\/jats:italic>\n            gene generates high-level discriminatory ability as well as fast, objective, reproducible, and portable data for epidemiological characterization of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            . Regarding putative antigenic epitopes of PorB for Genetic Systems monoclonal antibodies (MAbs), some of the previous findings were confirmed, but new findings were also observed. For several of the MAbs, however, the precise amino acid residues of PorB critical for single-MAb reactivity were difficult to identify. In addition, repeated serovar determination of 108\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            isolates revealed discrepancies for 34 isolates, mostly due to nonreproducible reactivity with single MAbs. Thus, the prospects of a genetic typing system with congruent translation of the serovar determination seem to be limited. In conclusion, analysis of short highly variable regions of the\n            <jats:italic>porB<\/jats:italic>\n            gene could form the basis for a fast molecular epidemiological tool for the examination of emergence and transmission of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            strains within the community.\n          <\/jats:p>","DOI":"10.1128\/jcm.41.9.4141-4147.2003","type":"journal-article","created":{"date-parts":[[2003,9,4]],"date-time":"2003-09-04T18:34:38Z","timestamp":1062700478000},"page":"4141-4147","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Comparison of Serologic and Genetic\n            <i>porB<\/i>\n            -Based Typing of\n            <i>Neisseria gonorrhoeae<\/i>\n            : Consequences for Future Characterization"],"prefix":"10.1128","volume":"41","author":[{"given":"Magnus","family":"Unemo","sequence":"first","affiliation":[{"name":"National Reference Laboratory for Pathogenic Neisseria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per","family":"Olce\u0301n","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Pathogenic Neisseria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Albert","sequence":"additional","affiliation":[{"name":"Department of Clinical Virology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Fredlund","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Pathogenic Neisseria"},{"name":"Unit for Infectious Disease Control, Department of Clinical Microbiology, O\u0308rebro University Hospital, O\u0308rebro"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-136-11-2165"},{"key":"e_1_3_2_3_2","first-page":"202","volume":"4","year":"1994","unstructured":"Chow, V. T. K., Q. C. Lau, and C. L. Poh. 1994. Mapping of serovar-specific monoclonal antibody epitopes by DNA and amino acid sequence analysis of Neisseria gonorrhoeae outer membrane protein IB strains. Immun. Infect. Dis.4:202-206.","journal-title":"Immun. Infect. Dis."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb12981.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-4-1415"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.5.2406-2413.1999"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.18.5591-5599.1999"},{"key":"e_1_3_2_9_2","first-page":"S12","volume":"74","year":"1998","unstructured":"Gerbase, A. C., J. T. Rowley, D. H. Heymann, S. F. Berkley, and P. Piot. 1998. Global prevalence and incidence estimates of selected curable STDs. Sex. Transm. Infect.74(suppl. 1):S12-S16.","journal-title":"Sex. Transm. Infect."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1994.tb06883.x"},{"key":"e_1_3_2_11_2","first-page":"53","volume":"67","year":"1991","unstructured":"Gill, M. J. 1991. Serotyping Neisseria gonorrhoeae: a report of the Fourth International Workshop. Genitourin. Med.67:53-57.","journal-title":"Genitourin. Med."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.22.8135"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/314608"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800050214"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/150.1.44"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1244"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-11-2613"},{"key":"e_1_3_2_18_2","first-page":"418","volume":"3","year":"1986","unstructured":"Nei, M., and T. Gojobori. 1986. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol. Biol. Evol.3:418-426.","journal-title":"Mol. Biol. Evol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.4.1039-1041.1995"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_17050865.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-43-3-201"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026322"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5375.363"},{"key":"e_1_3_2_24_2","first-page":"27","volume":"88","year":"1980","unstructured":"Sandstr\u00f6m, E., and D. Danielsson. 1980. Serology of Neisseria gonorrhoeae. Classification by co-agglutination. Acta Pathol. Microbiol. Scand. Sect. B88:27-38.","journal-title":"Acta Pathol. Microbiol. Scand. Sect. B"},{"key":"e_1_3_2_25_2","first-page":"363","volume":"12","year":"1995","unstructured":"Smith, N. H., J. Maynard Smith, and B. G. Spratt. 1995. Sequence evolution of the porB gene of Neisseria gonorrhoeae and Neisseria meningitidis: evidence of positive Darwinian selection. Mol. Biol. Evol.12:363-370.","journal-title":"Mol. Biol. Evol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.6.2335-2337.2001"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.36.3.1042-1053.1982"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1086\/315464"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.8.2914-2916.2000"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-200201000-00005"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3741-3749.2002"},{"key":"e_1_3_2_32_2","first-page":"569","volume":"10","year":"1994","unstructured":"van de Peer, Y., and R. De Wachter. 1994. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput. Appl. Biosci.10:569-570.","journal-title":"Comput. Appl. Biosci."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.9.2963-2971.1991"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.7.2183-2188.1999"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.12.4430-4438.2000"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.197-204.2003"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/149.2.166"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.9.4141-4147.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.41.9.4141-4147.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T02:26:20Z","timestamp":1646447180000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.41.9.4141-4147.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9]]},"references-count":36,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2003,9]]}},"alternative-id":["10.1128\/JCM.41.9.4141-4147.2003"],"URL":"https:\/\/doi.org\/10.1128\/jcm.41.9.4141-4147.2003","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,9]]},"assertion":[{"value":"2003-03-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-06-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}