{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T22:14:44Z","timestamp":1787782484309,"version":"build-2784847793"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Twelve isolates of\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            (1 of\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            , 8 of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , 1 of\n            <jats:italic>Proteus mirabilis<\/jats:italic>\n            , and 2 of\n            <jats:italic>Proteus vulgaris<\/jats:italic>\n            ) classified as extended-spectrum \u03b2-lactamase (ESBL) producers according to the ESBL screen flow application of the BD-Phoenix automatic system and for which the cefotaxime MICs were higher than those of ceftazidime were collected between January 2001 and July 2002 at the Laboratory of Clinical Microbiology of the San Matteo University Hospital of Pavia (northern Italy). By PCR and sequencing, a CTX-M-type determinant was detected in six isolates, including three of\n            <jats:italic>E. coli<\/jats:italic>\n            (carrying\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-1<\/jats:sub>\n            ), two of\n            <jats:italic>P. vulgaris<\/jats:italic>\n            (carrying\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-2<\/jats:sub>\n            ), and one of\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            (carrying\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            ). The three CTX-M-1-producing\n            <jats:italic>E. coli<\/jats:italic>\n            isolates were from different wards, and genotyping by pulsed-field gel electrophoresis (PFGE) revealed that they were clonally unrelated to each other. The two CTX-M-2-producing\n            <jats:italic>P. vulgaris<\/jats:italic>\n            isolates were from the same ward (although isolated several months apart), and PFGE analysis revealed probable clonal relatedness. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-1<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-2<\/jats:sub>\n            determinants were transferable to\n            <jats:italic>E. coli<\/jats:italic>\n            by conjugation, while conjugative transfer of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            determinant from\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            was not detectable. Present findings indicate that CTX-M enzymes of various types are present also in Italy and underscore that different CTX-M determinants can be found in a single hospital and can show different dissemination patterns. This is also the first report of CTX-M-2 in\n            <jats:italic>P. vulgaris<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.41.9.4264-4269.2003","type":"journal-article","created":{"date-parts":[[2003,9,4]],"date-time":"2003-09-04T18:34:38Z","timestamp":1062700478000},"page":"4264-4269","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":177,"title":["Multiple CTX-M-Type Extended-Spectrum \u03b2-Lactamases in Nosocomial Isolates of\n            <i>Enterobacteriaceae<\/i>\n            from a Hospital in Northern Italy"],"prefix":"10.1128","volume":"41","author":[{"given":"Laura","family":"Pagani","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze Morfologiche Eidologiche e Cliniche, Sezione di Microbiologia, Universita\u0300 di Pavia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Dell'Amico","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, I-53100 Siena"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Migliavacca","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Morfologiche Eidologiche e Cliniche, Sezione di Microbiologia, Universita\u0300 di Pavia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Maria","family":"D'Andrea","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, I-53100 Siena"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Giacobone","sequence":"additional","affiliation":[{"name":"Servizio di Analisi Microbiologiche IRCCS San Matteo, I-27100 Pavia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Amicosante","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche, Universita\u0300 di L'Aquila, I-67100 L'Aquila, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egidio","family":"Romero","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Morfologiche Eidologiche e Cliniche, Sezione di Microbiologia, Universita\u0300 di Pavia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, I-53100 Siena"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.7.2303-2306.2002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.1.151-159.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(92)90121-S"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01647010"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1936-1942.2000"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4030-4036.2002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.3.630-637.2002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.12.3595-3598.2001"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.7.2257-2261.2002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.2.534-537.2002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.3045-3049.2002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.4.867"},{"key":"e_1_3_2_15_2","first-page":"237","volume":"201","year":"2001","unstructured":"Karim, A., L. 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