{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T21:01:58Z","timestamp":1789506118689,"version":"build-2803163510"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Six independent isolates of an unusual black-pigmented\n            <jats:italic>Corynebacterium<\/jats:italic>\n            species (strains CN-1, CN-2, CN-3415, W70124, 91-0032, and 92-0360) were recovered from the human female urogenital tract. Four of the six source patients had complications of pregnancy, including spontaneous abortion, preterm labor, and low amniotic fluid volume at the time of the pathogen isolation. One isolate was recovered from a vaginal ulcer. All six strains yielded black-pigmented colonies on sheep blood agar, chocolate agar, and colistin-nalidixic acid agar after 24 to 48 h of incubation at 35\u00b0C. The dry, adherent colonies pitted the agar surface. The cells were coccobacillary to rod-shaped, catalase positive, nonmotile, and nonlipophilic. Only five of six isolates were available for characterization. Biochemical and chemotaxonomic studies revealed that the strains belong to the genus\n            <jats:italic>Corynebacterium<\/jats:italic>\n            but differ from known corynebacterial species. Comparative 16S rRNA gene sequence analysis showed that the strains are closely related and form a new subline within the genus\n            <jats:italic>Corynebacterium<\/jats:italic>\n            . We propose the name\n            <jats:italic>Corynebacterium nigricans<\/jats:italic>\n            sp. nov. for this group of coryneforms. The type strain of\n            <jats:italic>Corynebacterium nigricans<\/jats:italic>\n            is CN-1. It is deposited in the American Type Culture Collection (assigned strain number ATCC 700975) and in the Institute Pasteur collection (assigned strain number CIP 107346).\n          <\/jats:p>","DOI":"10.1128\/jcm.41.9.4353-4358.2003","type":"journal-article","created":{"date-parts":[[2003,9,4]],"date-time":"2003-09-04T18:34:38Z","timestamp":1062700478000},"page":"4353-4358","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["<i>Corynebacterium nigricans<\/i>\n            sp. nov.: Proposed Name for a Black-Pigmented\n            <i>Corynebacterium<\/i>\n            Species Recovered from the Human Female Urogenital Tract"],"prefix":"10.1128","volume":"41","author":[{"given":"Sanjay K.","family":"Shukla","sequence":"first","affiliation":[{"name":"Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn A.","family":"Bernard","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Harney","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, St. John's Hospital, Springfield, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel N.","family":"Frank","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt D.","family":"Reed","sequence":"additional","affiliation":[{"name":"Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.1.83-89.1991"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4375-4381.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/anae.2000.0408"},{"key":"e_1_3_2_5_2","unstructured":"Funke G. and K. A. Bernard. 2003. Coryneform gram-positive rods p. 472-501. In P. R. Murray E. J. Baron M. J. Pfaller F. C. Tenover and R. H. Holden (ed.) Manual of clinical microbiology 8th ed. ASM Press Washington D.C."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(97)00118-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.5.1124-1128.1996"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.3.624-627.1998"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-4399(98)80008-1"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-4399(98)80007-X"},{"key":"e_1_3_2_11_2","unstructured":"Lane D. J. 1991. 16S\/23S rRNA sequencing p. 115-175. In E. Stackebrandt and M. Goodfellow (ed.) Nucleic acid techniques in bacterial systematics. John Wiley & Sons Inc. New York N.Y."},{"key":"e_1_3_2_12_2","unstructured":"National Committee for Clinical Laboratory Standards. 1997. Minimum inhibitory concentration (MIC) interpretive standards (\u03bcg\/ml) for organisms other than Haemophilus spp. Neisseria gonorrhoeae and Streptococcus spp. NCCLS document M7-A4. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0602.000216"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199207303270501"},{"key":"e_1_3_2_15_2","unstructured":"Shukla S. K. M. Harney M. Jhaveri A. Andrews and K. D. Reed. Is a black-pigmented Corynebacterium species an opportunistic pathogen during pregnancy? Literature review and report of three new cases. Clin. Infect. Dis. in press."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.9.3346-3349.2002"},{"key":"e_1_3_2_17_2","first-page":"1701","volume":"6","year":"2000","unstructured":"Shukla, S. K., and K. D. Reed. 2000. Desulfovibrio desulfuricans bacteremia in a dog. J. Clin. 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Sinauer Associates Sunderland Mass."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1863-1870.1999"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.173.2.697-703.1991"},{"key":"e_1_3_2_24_2","first-page":"1001","year":"2002","unstructured":"Yassin, A. F., U. Steiner, and W. Ludwig. 2002. Corynebacterium aurimucosum sp. nov. and emended description of Corynebacterium minutissimum Collins and Jones (1983). Int. J. Syst. Evol. Microbiol. 52:1001-1005.","journal-title":"Int. J. Syst. 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