{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:13:34Z","timestamp":1761128014795},"reference-count":26,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            NCCLS screening and confirmation methods for detecting extended-spectrum \u03b2-lactamases (ESBLs) apply only to\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and\n            <jats:italic>Klebsiella<\/jats:italic>\n            spp., yet ESBLs have been found in other members of the family\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            . We evaluated the effectiveness of NCCLS methods for detecting ESBLs in 690 gram-negative isolates of\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            that excluded\n            <jats:italic>E. coli<\/jats:italic>\n            ,\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            , and\n            <jats:italic>Klebsiella oxytoca.<\/jats:italic>\n            Isolates were collected between January 1996 and June 1999 from 53 U.S. hospitals participating in Project ICARE (Intensive Care Antimicrobial Resistance Epidemiology). The antimicrobial susceptibility patterns of the isolates were determined by using the NCCLS broth microdilution method (BMD), and those isolates for which the MIC of ceftazidime, cefotaxime, ceftriaxone, or aztreonam was \u22652 \u03bcg\/ml or the MIC of cefpodoxime was \u22658 \u03bcg\/ml (positive ESBL screen test) were further tested for a clavulanic acid (CA) effect by BMD and the disk diffusion method (confirmation tests). Although 355 (51.4%) of the isolates were ESBL screen test positive, only 15 (2.2%) showed a CA effect. Since 3 of the 15 isolates were already highly resistant to the five NCCLS indicator drugs, ESBL detection would have an impact on the reporting of only 1.7% of the isolates in the study. Only 6 of the 15 isolates that showed a CA effect contained a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            , or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA<\/jats:sub>\n            \u03b2-lactamase gene as determined by PCR (with a corresponding isoelectric focusing pattern). Extension of the NCCLS guidelines for ESBL detection to\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            other than\n            <jats:italic>E. coli<\/jats:italic>\n            and\n            <jats:italic>Klebsiella<\/jats:italic>\n            spp. does not appear to be warranted in the United States at present, since the test has poor specificity for this population and would result in changes in categorical interpretations for only 1.7% of\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            tested.\n          <\/jats:p>","DOI":"10.1128\/jcm.42.1.294-298.2004","type":"journal-article","created":{"date-parts":[[2004,1,10]],"date-time":"2004-01-10T18:17:42Z","timestamp":1073758662000},"page":"294-298","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Utility of NCCLS Guidelines for Identifying Extended-Spectrum \u03b2-Lactamases in Non-\n            <i>Escherichia coli<\/i>\n            and Non-\n            <i>Klebsiella<\/i>\n            spp. of\n            <i>Enterobacteriaceae<\/i>"],"prefix":"10.1128","volume":"42","author":[{"given":"Mitchell J.","family":"Schwaber","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patti M.","family":"Raney","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Kamile","family":"Rasheed","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James W.","family":"Biddle","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Portia","family":"Williams","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Rollins School of Public Health, Emory University, Atlanta, Georgia 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"John E.","family":"McGowan","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Rollins School of Public Health, Emory University, Atlanta, Georgia 30322"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fred C.","family":"Tenover","sequence":"additional","affiliation":[{"name":"Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.8.2269-2275.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/319610"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/24.1.82"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.2.500-510.2002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.162.2.185"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.10.2593-2597.1997"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(00)00152-8"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01641355"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.557"},{"key":"e_1_3_2_13_2","unstructured":"Mabilat C. and S. Goussard. 1993. PCR detection and identification of genes for extended-spectrum \u03b2-lactamases p. 553-559. In D. H. Persing T. F. Smith F. C. Tenover and T. J. White (ed.) Diagnostic molecular microbiology: principles and applications. American Society for Microbiology Washington D.C."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-88-1-169"},{"key":"e_1_3_2_15_2","unstructured":"National Committee for Clinical Laboratory Standards. 2000. Performance standards for antimicrobial disk susceptibility tests 7th ed. Approved standard M2-A7. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_16_2","unstructured":"National Committee for Clinical Laboratory Standards. 2000. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically 5th ed. Approved standard M7-A5. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_17_2","unstructured":"National Committee for Clinical Laboratory Standards. 2002. Performance standards for antimicrobial susceptibility testing. Twelfth informational supplement M100-S12. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.5.1005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.11.2374"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.5.1462-1468.2002"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.6.1350"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.3.647"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1970-1973.2000"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.8.2864-2872.2001"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3142-3146.2003"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0702.010238"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0805.010346"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.42.1.294-298.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.42.1.294-298.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T13:05:37Z","timestamp":1646485537000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.42.1.294-298.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1128\/JCM.42.1.294-298.2004"],"URL":"https:\/\/doi.org\/10.1128\/jcm.42.1.294-298.2004","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"2003-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-10-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}