{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T23:24:28Z","timestamp":1793748268778,"version":"4.6.1"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Owing to the potential role of the tick\n                    <jats:italic>Amblyomma cooperi<\/jats:italic>\n                    in the enzootic cycle of\n                    <jats:italic>Rickettsia rickettsii<\/jats:italic>\n                    , the etiologic agent of Brazilian spotted fever (BSF), this study evaluated infection by\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    species in\n                    <jats:italic>A. cooperi<\/jats:italic>\n                    ticks collected from an area in Brazil where BSF is endemic. Among a total of 40\n                    <jats:italic>A. cooperi<\/jats:italic>\n                    adult ticks collected in an area of BSF endemicity in the state of Sa\u0303o Paulo, PCR analysis detected DNA of\n                    <jats:italic>Rickettsia bellii<\/jats:italic>\n                    in 16 ticks (40%), and 3 other ticks (7.5%) were positive for a previously unidentified spotted-fever-group (SFG) rickettsia. Cultivation in Vero cell cultures by the shell vial technique with individual\n                    <jats:italic>A. cooperi<\/jats:italic>\n                    ticks resulted in two isolates of\n                    <jats:italic>R. bellii<\/jats:italic>\n                    and one isolate genotypically characterized as an SFG rickettsia. The two\n                    <jats:italic>R. bellii<\/jats:italic>\n                    isolates were established in Vero cell cultures in the laboratory and were confirmed to be\n                    <jats:italic>R. bellii<\/jats:italic>\n                    by molecular analysis of the\n                    <jats:italic>gltA<\/jats:italic>\n                    and 17-kDa protein-encoding genes and by electron microscopic analysis. The SFG rickettsial isolate could not be stably passaged in cell culture in the laboratory, but molecular analysis of early passages suggested that it was closely related to\n                    <jats:italic>Rickettsia parkeri<\/jats:italic>\n                    ,\n                    <jats:italic>Rickettsia africae<\/jats:italic>\n                    , and\n                    <jats:italic>Rickettsia sibirica.<\/jats:italic>\n                    These results do not support the role of\n                    <jats:italic>A. cooperi<\/jats:italic>\n                    in the ecology of\n                    <jats:italic>R. rickettsii<\/jats:italic>\n                    in the area studied, but they add two more species of rickettsiae to the poorly developed list of species occurring in ticks in South America.\n                  <\/jats:p>","DOI":"10.1128\/jcm.42.1.90-98.2004","type":"journal-article","created":{"date-parts":[[2004,1,10]],"date-time":"2004-01-10T13:17:42Z","timestamp":1073740662000},"page":"90-98","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":556,"title":["<i>Rickettsia<\/i>\n                    Species Infecting\n                    <i>Amblyomma cooperi<\/i>\n                    Ticks from an Area in the State of Sa\u0303o Paulo, Brazil, Where Brazilian Spotted Fever Is Endemic"],"prefix":"10.1128","volume":"42","author":[{"given":"Marcelo B.","family":"Labruna","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas"},{"name":"Departamento de Medicina Veterinaria Preventiva e Saude Animal, Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ted","family":"Whitworth","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauri\u0301cio C.","family":"Horta","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterinaria Preventiva e Saude Animal, Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald H.","family":"Bouyer","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jere W.","family":"McBride","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano","family":"Pinter","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterinaria Preventiva e Saude Animal, Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vsevolod","family":"Popov","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solange M.","family":"Gennari","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterinaria Preventiva e Saude Animal, Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-2-339"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1970.19.1010"},{"key":"e_1_3_2_4_2","unstructured":"Burgdorfer W. 1988. 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