{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:21:58Z","timestamp":1791289318845,"version":"4.3.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We developed a new molecular tool based on\n            <jats:italic>rpoB<\/jats:italic>\n            gene (encoding the beta subunit of RNA polymerase) sequencing to identify streptococci. We first sequenced the complete\n            <jats:italic>rpoB<\/jats:italic>\n            gene for\n            <jats:italic>Streptococcus anginosus<\/jats:italic>\n            ,\n            <jats:italic>S. equinus<\/jats:italic>\n            , and\n            <jats:italic>Abiotrophia defectiva<\/jats:italic>\n            . Sequences were aligned with these of\n            <jats:italic>S. pyogenes<\/jats:italic>\n            ,\n            <jats:italic>S. agalactiae<\/jats:italic>\n            , and\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            available in GenBank. Using an in-house analysis program (SVARAP), we identified a 740-bp variable region surrounded by conserved, 20-bp zones and, by using these conserved zones as PCR primer targets, we amplified and sequenced this variable region in an additional 30\n            <jats:italic>Streptococcus<\/jats:italic>\n            ,\n            <jats:italic>Enterococcus<\/jats:italic>\n            ,\n            <jats:italic>Gemella<\/jats:italic>\n            ,\n            <jats:italic>Granulicatella<\/jats:italic>\n            , and\n            <jats:italic>Abiotrophia<\/jats:italic>\n            species. This region exhibited 71.2 to 99.3% interspecies homology. We therefore applied our identification system by PCR amplification and sequencing to a collection of 102 streptococci and 60 bacterial isolates belonging to other genera. Amplicons were obtained in streptococci and\n            <jats:italic>Bacillus cereus<\/jats:italic>\n            , and sequencing allowed us to make a correct identification of streptococci. Molecular signatures were determined for the discrimination of closely related species within the\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            -\n            <jats:italic>S. oralis<\/jats:italic>\n            -\n            <jats:italic>S. mitis<\/jats:italic>\n            group and the\n            <jats:italic>S. agalactiae<\/jats:italic>\n            -\n            <jats:italic>S. difficile<\/jats:italic>\n            group. These signatures allowed us to design a\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            -specific PCR and sequencing primer pair.\n          <\/jats:p>","DOI":"10.1128\/jcm.42.2.497-504.2004","type":"journal-article","created":{"date-parts":[[2004,2,6]],"date-time":"2004-02-06T18:43:10Z","timestamp":1076092990000},"page":"497-504","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":163,"title":["<i>rpoB<\/i>\n            Gene Sequence-Based Identification of Aerobic Gram-Positive Cocci of the Genera\n            <i>Streptococcus<\/i>\n            ,\n            <i>Enterococcus<\/i>\n            ,\n            <i>Gemella<\/i>\n            ,\n            <i>Abiotrophia<\/i>\n            , and\n            <i>Granulicatella<\/i>"],"prefix":"10.1128","volume":"42","author":[{"given":"Michel","family":"Drancourt","sequence":"first","affiliation":[{"name":"Unite\u0301 des Rickettsies, IFR 48, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ve\u0301ronique","family":"Roux","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, IFR 48, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Edouard","family":"Fournier","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, IFR 48, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, IFR 48, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Ayoub E. M. and P. Ferrieri. 1994. Group A streptococcal diseases p. 349-366. In P. D. Hoeprich M. C. Jordan and A. R. Ronald (ed.) Infectious diseases 5th ed. J. B. Lippincott Co. Philadelphia Pa."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(02)01382-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-35-6-367"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-41-4-487"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007203430303"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00285-6"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.5.1845-1847.2002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.512-513.2003"},{"key":"e_1_3_2_10_2","first-page":"365","year":"2000","unstructured":"Collins, M. D., and P. A. Lawson. 2000. 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