{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:23:14Z","timestamp":1792660994677,"version":"4.5.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Multiplex PCR was established for differential diagnosis of taeniasis and cysticercosis, including their causative agents. For identification of the parasites, multiplex PCR with cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    oxidase subunit 1 gene yielded evident differential products unique for\n                    <jats:italic>Taenia saginata<\/jats:italic>\n                    and\n                    <jats:italic>Taenia asiatica<\/jats:italic>\n                    and for American\/African and Asian genotypes of\n                    <jats:italic>Taenia solium<\/jats:italic>\n                    with molecular sizes of 827, 269, 720, and 984 bp, respectively. In the PCR-based detection of tapeworm carriers using fecal samples, the diagnostic markers were detected from 7 of 14 and 4 of 9\n                    <jats:italic>T. solium<\/jats:italic>\n                    carriers from Guatemala and Indonesia, respectively. Test sensitivity may have been reduced by the length of time (up to 12 years) that samples were stored and\/or small sample volumes (ca. 30 to 50 mg). However, the diagnostic markers were detected by nested PCR in five worm carriers from Guatemalan cases that were found to be negative by multiplex PCR. It was noteworthy that a 720 bp-diagnostic marker was detected from a\n                    <jats:italic>T. solium<\/jats:italic>\n                    carrier who was egg-free, implying that it is possible to detect worm carriers and treat before mature gravid proglottids are discharged. In contrast to\n                    <jats:italic>T. solium<\/jats:italic>\n                    carriers, 827-bp markers were detected by multiplex PCR in all\n                    <jats:italic>T. saginata<\/jats:italic>\n                    carriers. The application of the multiplex PCR would be useful not only for surveillance of taeniasis and cysticercosis control but also for the molecular epidemiological survey of these cestode infections.\n                  <\/jats:p>","DOI":"10.1128\/jcm.42.2.548-553.2004","type":"journal-article","created":{"date-parts":[[2004,2,6]],"date-time":"2004-02-06T13:43:10Z","timestamp":1076074990000},"page":"548-553","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":199,"title":["DNA Differential Diagnosis of Taeniasis and Cysticercosis by Multiplex PCR"],"prefix":"10.1128","volume":"42","author":[{"given":"Hiroshi","family":"Yamasaki","sequence":"first","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Allan","sequence":"additional","affiliation":[{"name":"Pfizer Animal Health Business Development, Pfizer, Ltd., Sandwich"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello Otake","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Nakao","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhito","family":"Sako","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Nakaya","sequence":"additional","affiliation":[{"name":"Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongchuan","family":"Qiu","sequence":"additional","affiliation":[{"name":"Sichuan Institute of Parasitic Diseases, Chengdu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wulamu","family":"Mamuti","sequence":"additional","affiliation":[{"name":"Department of Parasitology"},{"name":"Department of Parasitology, Xinjiang Medical University, Urumqi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip S.","family":"Craig","sequence":"additional","affiliation":[{"name":"Bioscience Research Institute, School of Environment and Life Sciences, University of Salford, Greater Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000060686"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1996.11813039"},{"key":"e_1_3_2_4_2","first-page":"33","volume":"50","year":"1994","unstructured":"Bowles, J., and D. P. McManus. 1994. Genetic characterization of the Asian Taenia, a newly described taeniid cestode of humans. Am. J. Trop. Med. Hyg.50:33-44.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.5.1283-1288.1995"},{"key":"e_1_3_2_6_2","first-page":"131","volume":"4","year":"1998","unstructured":"Flisser, A. 1998. Neurocysticercosis in Mexico. Parasitol. Today4:131-137.","journal-title":"Parasitol. Today"},{"key":"e_1_3_2_7_2","first-page":"1429","year":"1988","unstructured":"Flisser, A., A. Reid, Z. E. Garcia, and D. P. McManus. 1988. Specific detection of Taenia saginata eggs by DNA hybridization. Lancetii:1429-1430.","journal-title":"Lancet"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.737-744.2000"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(01)00356-X"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000078768"},{"key":"e_1_3_2_11_2","first-page":"83","volume":"32","year":"2001","unstructured":"Ito, A., K. Nakaya, Y. Sako, M. Nakao, and M. Ito. 2001. NOD-scid mouse as an experimental animal model for cysticercosis. Southeast Asian J. Trop. Med. Public Health32(Suppl. 2):83-89.","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(03)00200-9"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(02)00269-3"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.133-137.2000"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002001725"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60176-1"},{"key":"e_1_3_2_17_2","first-page":"1275","year":"1987","unstructured":"Rishi, A. K., and D. P. McManus. 1987. DNA probes which unambiguously distinguish Taenia solium from T. saginata.Lanceti:1275-1276.","journal-title":"Lancet"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200006683X"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2002)088[1007:COCTTD]2.0.CO;2"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1992.46.677"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(97)01104-6"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","unstructured":"Wouters G. J. Brandt and S. Geerts. 1987. Observations on possible strain differences in Taenia saginata p. 76-80. In S. Geerts V. Kumar and J. Brandt (ed.) Helminth zoonoses. Martinus Nijhoff Publishers Dordrecht The Netherlands.","DOI":"10.1007\/978-94-009-3341-5_12"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3818-3821.2002"},{"key":"e_1_3_2_24_2","unstructured":"Yamasaki H. M. Nakao Y. Sako K. Nakaya M. O. Sato W. Mamuti N. Xiao M. Okamoto and A. Ito. 2002. DNA Differential diagnosis of human taeniid cestodes using restriction fragment length polymorphism and base excision sequence scanning T-base system p. 189-193. In Proceedings of the 10th International Congress of Parasitology. Monduzzi Editore Boronia Italy."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.42.2.548-553.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.42.2.548-553.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T21:51:57Z","timestamp":1646430717000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.42.2.548-553.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,2]]}},"alternative-id":["10.1128\/JCM.42.2.548-553.2004"],"URL":"https:\/\/doi.org\/10.1128\/jcm.42.2.548-553.2004","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2]]},"assertion":[{"value":"2003-08-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-11-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}