{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:44:18Z","timestamp":1791179058163,"version":"4.1.0"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cell-to-cell signaling involving\n            <jats:italic>N<\/jats:italic>\n            -acyl-homoserine lactone compounds termed autoinducers (AIs) is instrumental to virulence factor production and biofilm development by\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            . In order to determine the importance of cell-to-cell signaling during the colonization of mechanically ventilated patients, we collected 442\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            pulmonary isolates from 13 patients. Phenotypic characterization showed that 81% of these isolates produced the AI-dependent virulence factors elastase, protease, and rhamnolipids. We identified nine genotypically distinct\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            strains. Six of these strains produced AIs [\n            <jats:italic>N<\/jats:italic>\n            -butanoyl-homoserine lactone or\n            <jats:italic>N<\/jats:italic>\n            -(3-oxo-dodecanoyl)-homoserine lactone] and extracellular virulence factors (elastase, total exoprotease, rhamnolipid, hydrogen cyanide, or pyocyanin) in vitro. Three of the nine strains were defective in the production of both AIs and extracellular virulence factors. Two of these strains had mutational defects in both the\n            <jats:italic>lasR<\/jats:italic>\n            and\n            <jats:italic>rhlR<\/jats:italic>\n            genes, which encode the\n            <jats:italic>N<\/jats:italic>\n            -acyl-homoserine lactone-dependent transcriptional regulators LasR and RhlR, respectively. The third of these AI-deficient strains was only mutated in the\n            <jats:italic>lasR<\/jats:italic>\n            gene. Our observations suggest that most, but not all, strains colonizing intubated patients are able to produce virulence factors and that mutations affecting the cell-to-cell signaling circuit are preferentially located in the transcriptional regulator genes.\n          <\/jats:p>","DOI":"10.1128\/jcm.42.2.554-562.2004","type":"journal-article","created":{"date-parts":[[2004,2,6]],"date-time":"2004-02-06T18:43:10Z","timestamp":1076092990000},"page":"554-562","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":69,"title":["Characterization of Cell-to-Cell Signaling-Deficient\n            <i>Pseudomonas aeruginosa<\/i>\n            Strains Colonizing Intubated Patients"],"prefix":"10.1128","volume":"42","author":[{"given":"Vale\u0301rie","family":"De\u0301nervaud","sequence":"first","affiliation":[{"name":"Institut de Microbiologie Fondamentale, Universite\u0301 de Lausanne, CH-1015 Lausanne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"TuQuoc","sequence":"additional","affiliation":[{"name":"Division des Maladies Infectieuses, Ho\u0302pital Universitaire de Gene\u0300ve, CH-1211 Geneva 14"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Blanc","sequence":"additional","affiliation":[{"name":"Division Autonome de Me\u0301decine Pre\u0301ventive, Centre Hospitalier Vaudois, CH-1011 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Favre-Bonte\u0301","sequence":"additional","affiliation":[{"name":"Division des Maladies Infectieuses, Ho\u0302pital Universitaire de Gene\u0300ve, CH-1211 Geneva 14"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viji","family":"Krishnapillai","sequence":"additional","affiliation":[{"name":"Institut de Microbiologie Fondamentale, Universite\u0301 de Lausanne, CH-1015 Lausanne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Reimmann","sequence":"additional","affiliation":[{"name":"Institut de Microbiologie Fondamentale, Universite\u0301 de Lausanne, CH-1015 Lausanne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Haas","sequence":"additional","affiliation":[{"name":"Institut de Microbiologie Fondamentale, Universite\u0301 de Lausanne, CH-1015 Lausanne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"van Delden","sequence":"additional","affiliation":[{"name":"Division des Maladies Infectieuses, Ho\u0302pital Universitaire de Gene\u0300ve, CH-1211 Geneva 14"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90604-A"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0580-9517(08)70662-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.24.7155-7163.1995"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1139\/m75-088"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.1998.11.11.1119"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.108.2_Supplement.1S"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5361.295"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.4839-4849.2000"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.20.9878"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.1783-1790.2002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.2.884-900.1990"},{"key":"e_1_3_2_14_2","first-page":"221","volume":"70","year":"1990","unstructured":"Farinha, M. 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