{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T16:26:28Z","timestamp":1694622388464},"reference-count":24,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A mismatch amplification mutation assay (MAMA) was developed for identification of point mutations in quinolone resistance-determining region (QRDR) of\n            <jats:italic>gyrA<\/jats:italic>\n            at codons 91 and 95. MAMA PCR was used to detect mutations at codons 91 and 95 of\n            <jats:italic>gyrA<\/jats:italic>\n            in 117\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            isolates (with ciprofloxacin MICs of 0.004 to &gt;32 \u03bcg\/ml) from Bangladesh during 1997 to 2001. The QRDR regions of the\n            <jats:italic>gyrA<\/jats:italic>\n            genes from 31 randomly selected isolates were sequenced, and the results were compared with those of MAMA PCR. Using mismatch PCR, a mutation at Ser91 could be detected in all 27 (resistant and intermediate) isolates, and an Asp95-to-Gly95 mutation could be detected in all 15 isolates, as detected by sequencing. MAMA PCR offers a simple, inexpensive, rapid, and easier alternative for detection of point mutations in fluoroquinolone resistance in\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.42.2.591-594.2004","type":"journal-article","created":{"date-parts":[[2004,2,6]],"date-time":"2004-02-06T18:43:10Z","timestamp":1076092990000},"page":"591-594","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of Mismatch Amplification Mutation Assay with DNA Sequencing for Characterization of Fluoroquinolone Resistance in\n            <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1128","volume":"42","author":[{"given":"Zafar","family":"Sultan","sequence":"first","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1000, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shamsun","family":"Nahar","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1000, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengt","family":"Wretlind","sequence":"additional","affiliation":[{"name":"Division of Clinical Bacteriology, Huddinge University Hospital, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Lindback","sequence":"additional","affiliation":[{"name":"Division of Clinical Bacteriology, Huddinge University Hospital, Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motiur","family":"Rahman","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1000, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01297.x"},{"key":"e_1_3_2_3_2","first-page":"1","volume":"51","year":"2002","unstructured":"Centers for Disease Control and Prevention. 2002. 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