{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:15:30Z","timestamp":1767626130587},"reference-count":28,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The inflammatory bowel diseases are considered an abnormal host immune response to an environmental stimulus. Evidence suggests a role for intestinal bacteria in initiating and\/or providing an ongoing stimulus for inflammation in inflammatory bowel disease.\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            is the major cause of active chronic gastritis and peptic ulcers in humans and has been linked to gastric carcinoma and lymphoma. Studies in various animal models, particularly mice, have identified enterohepatic\n            <jats:italic>Helicobacter<\/jats:italic>\n            species that are capable of causing hepatitis and enterocolitis. We hypothesize that\n            <jats:italic>Helicobacter<\/jats:italic>\n            species may have a role in maintaining inflammation in humans with inflammatory bowel disease. In order to investigate this, biopsy specimens were obtained from patients with and without inflammatory bowel disease. DNA was extracted from the tissues and subjected to PCR with primers designed to detect the ribosomal DNA of members of the\n            <jats:italic>Helicobacter<\/jats:italic>\n            species. DNA from six biopsy samples from 60 inflammatory bowel disease patients tested positive. This included 5 of 33 ulcerative colitis patients that were positive compared to 0 of 29 age-matched controls (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.04). Sequencing of the bands produced by PCR amplification revealed \u226599% homology with\n            <jats:italic>H. pylori<\/jats:italic>\n            . These results indicate that a member of the\n            <jats:italic>Helicobacter<\/jats:italic>\n            species may be involved in some cases of ulcerative colitis.\n          <\/jats:p>","DOI":"10.1128\/jcm.42.2.660-664.2004","type":"journal-article","created":{"date-parts":[[2004,2,6]],"date-time":"2004-02-06T18:43:10Z","timestamp":1076092990000},"page":"660-664","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Detection of Species-Specific\n            <i>Helicobacter<\/i>\n            Ribosomal DNA in Intestinal Biopsy Samples from a Population-Based Cohort of Patients with Ulcerative Colitis"],"prefix":"10.1128","volume":"42","author":[{"given":"C. J.","family":"Streutker","sequence":"first","affiliation":[{"name":"Intestinal Disease Research Unit"},{"name":"Department of Laboratory and Molecular Medicine"},{"name":"Department of Medicine, McMaster University, Hamilton"},{"name":"Department of Pathology and Laboratory Medicine, St. Michael's Hospital, and Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. N.","family":"Bernstein","sequence":"additional","affiliation":[{"name":"Department of Medicine"},{"name":"Inflammatory Bowel Disease Clinical and Research Centre, University of Manitoba, Winnipeg"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. L.","family":"Chan","sequence":"additional","affiliation":[{"name":"Departments of Medical Genetics and Microbiology and Laboratory Medicine and Pathobiology, University of Toronto, Toronto"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. H.","family":"Riddell","sequence":"additional","affiliation":[{"name":"Intestinal Disease Research Unit"},{"name":"Department of Laboratory and Molecular Medicine"},{"name":"Department of Pathology, Mount Sinai Hospital, Toronto, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Croitoru","sequence":"additional","affiliation":[{"name":"Intestinal Disease Research Unit"},{"name":"Department of Medicine, McMaster University, Hamilton"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"313","volume":"31","year":"1999","unstructured":"Amati, L., L. Caradonna, E. Jirillo, and D. Caccavo. 1999. Immunological disorders in inflammatory bowel disease and immunotherapeutic implications. Ital. J. Gastroenterol. Hepatol.31:313-325.","journal-title":"Ital. J. Gastroenterol. 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