{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:20:31Z","timestamp":1791570031612,"version":"4.3.4"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Mycoplasma genitalium<\/jats:italic>\n            is a cause of nongonococcal urethritis, particularly in patients not infected with\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            . A quantitative 5\u2032 nuclease assay (TaqMan PCR) was developed and validated. The assay detected a fragment of the MgPa adhesin gene by use of a TaqMan MGB (minor groove binder) probe and included an internal processing control to detect PCR inhibition. Urethral swab specimens and first-void urine samples from\n            <jats:italic>M. genitalium<\/jats:italic>\n            -positive men were examined, and the\n            <jats:italic>M. genitalium<\/jats:italic>\n            DNA load was correlated to symptoms and signs. The assay consistently detected &lt;5 genome copies without cross-reactions with other mycoplasmas. Urine and urethral swab specimens from men with urethritis had higher\n            <jats:italic>M. genitalium<\/jats:italic>\n            DNA loads than specimens from men without urethritis. However, a very broad overlap of DNA loads between patients with and without urethritis was observed. Urethral swab specimens from patients with urethral discharge had a significantly higher DNA load than specimens from patients without discharge. This correlation was not found in first-void urine specimens.\n          <\/jats:p>","DOI":"10.1128\/jcm.42.2.683-692.2004","type":"journal-article","created":{"date-parts":[[2004,2,6]],"date-time":"2004-02-06T18:43:10Z","timestamp":1076092990000},"page":"683-692","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":273,"title":["Use of TaqMan 5\u2032 Nuclease Real-Time PCR for Quantitative Detection of\n            <i>Mycoplasma genitalium<\/i>\n            DNA in Males with and without Urethritis Who Were Attendees at a Sexually Transmitted Disease Clinic"],"prefix":"10.1128","volume":"42","author":[{"given":"J\u00f8rgen Skov","family":"Jensen","sequence":"first","affiliation":[{"name":"Mycoplasma Laboratory, Statens Serum Institut, DK-2300 Copenhagen S, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Bjo\u0308rnelius","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology, Huddinge University Hospital, Karolinska Institutet, S-14 186 Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birthe","family":"Dohn","sequence":"additional","affiliation":[{"name":"Mycoplasma Laboratory, Statens Serum Institut, DK-2300 Copenhagen S, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Lidbrink","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology, Huddinge University Hospital, Karolinska Institutet, S-14 186 Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.11.2266-2269.1988"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/095646240001100504"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3854-3856.2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.79.4.318"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-200302000-00007"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)91411-E"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1258\/095646202760326408"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","unstructured":"Jensen J. 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