{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T22:20:00Z","timestamp":1767824400415,"version":"3.49.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2004,3,1]],"date-time":"2004-03-01T00:00:00Z","timestamp":1078099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have recently identified a 150-kDa surface antigen of\n            <jats:italic>Entamoeba histolytica<\/jats:italic>\n            as an intermediate subunit (Igl) of galactose- and\n            <jats:italic>N<\/jats:italic>\n            -acetyl\n            <jats:sc>-d-<\/jats:sc>\n            galactosamine-inhibitable lectin, which is a cysteine-rich protein consisting of 1,101 amino acids (aa) and containing multiple CXXC motifs in amino acid sequences. In the present study, full-length Igl except for the signal sequences (aa 14 to 1088) and three fragments of Igl\u2014the N-terminal part (aa 14 to 382), the middle part (aa 294 to 753), and the C-terminal part (aa 603 to 1088)\u2014were prepared in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , and the reactivity of these recombinant proteins with sera from patients with amebiasis was examined by means of enzyme-linked immunosorbent assay (ELISA). Sera from 57 symptomatic patients with amebic liver abscess or amebic colitis, sera from 15 asymptomatic cyst passers, sera from 40 individuals with other protozoan infections, and sera from 50 healthy controls were used. The sensitivity and specificity of the recombinant full-length Igl in the ELISA were 90 and 94%, respectively. When three fragments were used as antigens in the ELISA, the sensitivities were 56% in the N terminus, 92% in the middle part, and 97% in the C terminus. The specificities of the three antigens were 96% in the N terminus and 99% in both the middle and C-terminal fragments. These results demonstrate that Igl is well recognized in not only symptomatic but also asymptomatic patients with\n            <jats:italic>E. histolytica<\/jats:italic>\n            infection and that the carboxyl terminus of Igl is an especially useful antigen for the serodiagnosis of amebiasis.\n          <\/jats:p>","DOI":"10.1128\/jcm.42.3.1069-1074.2004","type":"journal-article","created":{"date-parts":[[2004,3,5]],"date-time":"2004-03-05T23:57:55Z","timestamp":1078531075000},"page":"1069-1074","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Evaluation of Recombinant Fragments of\n            <i>Entamoeba histolytica<\/i>\n            Gal\/GalNAc Lectin Intermediate Subunit for Serodiagnosis of Amebiasis"],"prefix":"10.1128","volume":"42","author":[{"given":"Hiroshi","family":"Tachibana","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xun-Jia","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa"},{"name":"Department of Medical Microbiology and Parasitology, Fudan University School of Medicine, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gohta","family":"Masuda","sequence":"additional","affiliation":[{"name":"Tokyo Metropolitan Komagome Hospital, Bunkyo-ku"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyuki","family":"Horiki","sequence":"additional","affiliation":[{"name":"Division of Internal Medicine, St. Luke's International Hospital, Chuo-ku"},{"name":"Third Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine and Parasitology, Keio University School of Medicine, Shinjuku-ku, Tokyo"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.1.109"},{"key":"e_1_3_2_3_2","first-page":"13","volume":"18","year":"1997","unstructured":"Anonymous. 1997. WHO\/PAHO\/UNESCO report: a consultation with experts on amoebiasis. Epidemiol. Bull.18:13-14.","journal-title":"Epidemiol. Bull."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4894(02)00113-3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5892-5898.2001"},{"key":"e_1_3_2_6_2","first-page":"159","volume":"25","year":"1997","unstructured":"Cheng, X. J., Y. Kaneda, and H. Tachibana. 1997. A monoclonal antibody against the 150-kDa surface antigen of Entamoeba histolytica inhibits adherence and cytotoxicity to mammalian cells. Med. Sci. Res.25:159-161.","journal-title":"Med. Sci. 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