{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:30:08Z","timestamp":1791412208535,"version":"4.3.3"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2004,6,1]],"date-time":"2004-06-01T00:00:00Z","timestamp":1086048000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A real-time PCR assay was developed to diagnose and identify the causative agents of suspected mycobacterial lymphadenitis. Primers and probes for the real-time PCR were designed on the basis of the internal transcribed spacer sequence, enabling the recognition of the genus\n            <jats:italic>Mycobacterium<\/jats:italic>\n            and the species\n            <jats:italic>Mycobacterium avium<\/jats:italic>\n            and\n            <jats:italic>M<\/jats:italic>\n            .\n            <jats:italic>tuberculosis<\/jats:italic>\n            . The detection limit for the assay was established at 1,100 CFU\/ml of pus, and the specificity tests showed no false-positive reaction with other mycobacterial species and other pathogens causing lymphadenitis. From 67 children with suspected mycobacterial lymphadenitis based on a positive mycobacterial skin test, 102 samples (58 fine-needle aspirates [FNA] and 44 tissue specimens) were obtained. The real-time PCR assay detected a mycobacterial infection in 48 patients (71.6%), whereas auramine staining and culturing were positive for 31 (46.3%) and 28 (41.8%) of the patients. The addition of the real-time PCR assay to conventional diagnostic tests resulted in the recognition of 13 more patients with mycobacterial disease. These results indicate that the real-time PCR is more sensitive than conventional staining and culturing techniques (\n            <jats:italic>P<\/jats:italic>\n            = 0.006). The\n            <jats:italic>M<\/jats:italic>\n            .\n            <jats:italic>avium<\/jats:italic>\n            -specific real-time PCR was positive for 38 patients, and the\n            <jats:italic>M<\/jats:italic>\n            .\n            <jats:italic>tuberculosis<\/jats:italic>\n            -specific real-time PCR was positive for 1 patient. Analysis of 27 patients from whom FNA and tissue biopsy specimens were collected revealed significantly more positive real-time PCR results for FNA than for tissue biopsy specimens (\n            <jats:italic>P<\/jats:italic>\n            = 0.003). Samples from an age-matched control group of 50 patients with PCR-proven cat scratch disease were all found to be negative by the real-time PCR. We conclude that this real-time PCR assay with a sensitivity of 72% for patients with lymphadenitis and a specificity of 100% for the detection of atypical mycobacteria can provide excellent support for clinical decision making in children with lymphadenitis.\n          <\/jats:p>","DOI":"10.1128\/jcm.42.6.2644-2650.2004","type":"journal-article","created":{"date-parts":[[2004,6,7]],"date-time":"2004-06-07T18:09:38Z","timestamp":1086631778000},"page":"2644-2650","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":125,"title":["Real-Time PCR Assay Using Fine-Needle Aspirates and Tissue Biopsy Specimens for Rapid Diagnosis of Mycobacterial Lymphadenitis in Children"],"prefix":"10.1128","volume":"42","author":[{"given":"E. S.","family":"Bruijnesteijn van Coppenraet","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Lindeboom","sequence":"additional","affiliation":[{"name":"Departments of Oral and Maxillofacial Surgery"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Prins","sequence":"additional","affiliation":[{"name":"Internal Medicine, Academic Medical Centre, Amsterdam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. F.","family":"Peeters","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, St. Elisabeth Hospital, Tilburg, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. C. J.","family":"Claas","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. J.","family":"Kuijper","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.156.1.ed08-97"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(96)70288-3"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/171.4.916"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.495-503.1990"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.1.198-204.2002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/080352501317130434"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.9.2.177"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.72.6.516"},{"key":"e_1_3_2_10_2","first-page":"399","volume":"36","year":"1989","unstructured":"Hansen, K. N., I. Heltberg, and K. Hjelt. 1989. Sensitivity to tuberculin and sensitins from atypical mycobacteria (M. chelonae subsp. abscessus, M. avium, M. intracellulare, M. scrofulaceum) in 100 Danish school children. Dan. Med. Bull.36:399-401.","journal-title":"Dan. Med. Bull."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/515091"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-4362.2003.01461.x"},{"key":"e_1_3_2_13_2","first-page":"775","volume":"87","year":"1963","unstructured":"Kubica, G. P., W. E. Dye, M. L. Cohn, and G. Middlebrook. 1963. Sputum digestion and decontamination with N-acetyl-l-cysteine-sodium hydroxide for culture of mycobacteria. Am. Rev. Respir. Dis.87:775-779.","journal-title":"Am. Rev. Respir. Dis."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000276704"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5876(01)00558-4"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.9.3364-3373.2002"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-3879(91)90021-J"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(99)70294-5"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-002-0825-y"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4143-4147.2002"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200110000-00024"},{"key":"e_1_3_2_22_2","first-page":"1829","volume":"20","year":"1999","unstructured":"Robson, C., R. Hazra, P. D. Barnes, R. L. Robertson, D. Jones, and R. N. Husson. 1999. Nontuberculous mycobacterial infection of the head and neck in immunocompetent children: CT and MR findings. Am. J. Neuroradiol.20:1829-1835.","journal-title":"Am. J. Neuroradiol."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8479(05)80028-0"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.1.139-147.1998"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1094-1104.2000"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","unstructured":"Rozen S. and H. J. Skaletsky. 2000. Primer3 on the WWW for general users and for biologist programmers p. 365-386. In S. Krawetz and S. Misener (ed.) Bioinformatics methods and protocols: methods in molecular biology. Humana Press Totowa N.J.","DOI":"10.1385\/1-59259-192-2:365"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4366-4371.2003"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199602)178:2<221::AID-PATH441>3.0.CO;2-W"},{"key":"e_1_3_2_29_2","first-page":"1367","volume":"147","year":"2003","unstructured":"van de Griendt, E. J., P. J. Rietra, and R. N. van Andel. 2003. Mycobacterium haemophilum als veroorzaker van lymphadenitis colli bij een niet-zieke jongen Ned. Tijdschr. Geneeskd.147:1367-1369.","journal-title":"Tijdschr. Geneeskd."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/19.1.15"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1086\/514225"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.6.1385-1389.1997"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.42.6.2644-2650.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.42.6.2644-2650.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T03:11:05Z","timestamp":1646449865000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.42.6.2644-2650.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["10.1128\/JCM.42.6.2644-2650.2004"],"URL":"https:\/\/doi.org\/10.1128\/jcm.42.6.2644-2650.2004","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,6]]},"assertion":[{"value":"2003-10-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-02-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}