{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T21:10:30Z","timestamp":1793740230962,"version":"4.6.1"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2004,7,1]],"date-time":"2004-07-01T00:00:00Z","timestamp":1088640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2% produced ESBLs. ESBL-producing isolates were detected in every region in Korea. A total of 44.6% of the isolates produced both TEM- and SHV-type ESBLs, and 52% of ESBL-producing isolates transferred resistance to ceftazidime by transconjugation. The ESBLs were TEM-19, TEM-20, TEM-52, SHV-2a, SHV-12, and one new variant identified for the first time in Korea, namely, TEM-116. TEM-1 and SHV-12 were by far the most common variants. TEM-1, TEM-116, and SHV-12 showed a high prevalence in\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            . Two isolates (\n            <jats:italic>E. coli<\/jats:italic>\n            SH16 and\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            SV3) produced CMY-1-like beta-lactamases, which play a decisive role in resistance to cefoxitin and cefotetan, as well as TEM-type enzymes (TEM-20 and TEM-52, respectively). Using MIC patterns and DNA sequencing analysis, we postulated a possible evolution scheme among TEM-type beta-lactamases in Korea: from TEM-1 to TEM-19, from TEM-19 to TEM-20, and from TEM-20 to TEM-52.\n          <\/jats:p>","DOI":"10.1128\/jcm.42.7.2902-2906.2004","type":"journal-article","created":{"date-parts":[[2004,7,8]],"date-time":"2004-07-08T18:19:31Z","timestamp":1089310771000},"page":"2902-2906","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["Molecular Characterization of Extended-Spectrum Beta-Lactamases Produced by Clinical Isolates of\n            <i>Klebsiella pneumoniae<\/i>\n            and\n            <i>Escherichia coli<\/i>\n            from a Korean Nationwide Survey"],"prefix":"10.1128","volume":"42","author":[{"given":"Seok Hoon","family":"Jeong","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Kosin University College of Medicine, Busan 602-702"},{"name":"Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Il Kwon","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Kosin University College of Medicine, Busan 602-702"},{"name":"Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Hun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Myongji University, Yongin, Kyunggido 449-728"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Ghyu","family":"Sohn","sequence":"additional","affiliation":[{"name":"Bio Technology Innovation Center, Youngdong University, Chungbuk 370-701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geun Ho","family":"Kang","sequence":"additional","affiliation":[{"name":"Bio Technology Innovation Center, Youngdong University, Chungbuk 370-701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghil Ja","family":"Jeon","sequence":"additional","affiliation":[{"name":"Bio Technology Innovation Center, Youngdong University, Chungbuk 370-701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Bio Technology Innovation Center, Youngdong University, Chungbuk 370-701"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byeong Chul","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Myongji University, Yongin, Kyunggido 449-728"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Myongji University, Yongin, Kyunggido 449-728"},{"name":"Bio Technology Innovation Center, Youngdong University, Chungbuk 370-701"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.8.1926"},{"key":"e_1_3_2_3_2","first-page":"464","volume":"38","year":"1990","unstructured":"Ben, H. A., G. Fournier, A. Kechird, C. Fendri, R. S. Ben, and A. Phillippon. 1990. Enzymatic resistance to cefotaxime in 56 strains of Klebsiella spp., Escherichia coli and Salmonella spp. at a Tunisian Hospital (1984-1988). Pathol. Biol. (Paris)38:464-469.","journal-title":"Pathol. Biol. (Paris)"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.6.839"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/35.1.7"},{"key":"e_1_3_2_7_2","unstructured":"Farmer J. J. III. 1995. Enterobacteriaceae : introduction and identification p. 438-449. In P. R. Murray E. J. Baron M. A. Pfaller F. C. Tenover and R. H. Yolken (ed.) Manual of clinical microbiology 3rd ed. American Society for Microbiology Washington D.C."},{"key":"e_1_3_2_8_2","first-page":"101","volume":"45","year":"2000","unstructured":"Giakkoupi, P., E. Tzelepi, P. T. Tassios, N. T. Legakis, and L. S. Tzouvelekis. 2000. Determinational effect of the combination of R164S with G238S in TEM-1 beta-lactamase on the extended-spectrum activity conferred by each single mutation. J. Antimicrob. Chemother.45:101-104.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.3035-3038.2002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.2001.0987"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.4.867"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.5.1446-1449.1998"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.2.269"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765x.2000.00806.x"},{"key":"e_1_3_2_15_2","first-page":"157","volume":"200","year":"2001","unstructured":"Lee, S. H., J. Y. Kim, S. H. Shin, S. K. Lee, M. M. Choi, I. Y. Lee, Y. B. Kim, J. Y. Cho, W. Jin, and K. J. Lee. 2001. Restriction fragment length dimorphism-PCR method for the detection of extended-spectrum beta-lactamases unrelated to TEM- and SHV-types. FEMS Microbiol. Lett.200:157-161.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.557"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.2.464"},{"key":"e_1_3_2_18_2","unstructured":"National Committee for Clinical Laboratory Standards. 1999. Performance standards for antimicrobial susceptibility testing 9th ed. Informational supplement. M100-S9. 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