{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:07:31Z","timestamp":1790968051801,"version":"4.1.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2004,7,1]],"date-time":"2004-07-01T00:00:00Z","timestamp":1088640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We evaluated the Hyplex BloodScreen PCR-enzyme-linked immunosorbent assay (ELISA) system (BAG, Lich, Germany), a new diagnostic test for the direct identification of gram-negative bacilli and gram-positive cocci from positive blood cultures, with 482 positive BACTEC 9240 blood culture bottles. The test involves amplification of the bacterial DNA by multiplex PCR and subsequent hybridization of the PCR product to specific oligonucleotide probes in an ELISA-based format. The available probes allow the separate detection of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            ,\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            ,\n            <jats:italic>Enterobacter aerogenes<\/jats:italic>\n            ,\n            <jats:italic>Klebsiella<\/jats:italic>\n            spp.,\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            ,\n            <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n            ,\n            <jats:italic>Enterococcus faecalis<\/jats:italic>\n            \/\n            <jats:italic>Enterococcus faecium<\/jats:italic>\n            ,\n            <jats:italic>Streptococcus pyogenes<\/jats:italic>\n            , and\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            and the staphylococcal\n            <jats:italic>mecA<\/jats:italic>\n            gene. The Hyplex BloodScreen test showed an overall sensitivity of 100% for the identification of gram-negative bacilli and 96.6 to 100% for the identification of gram-positive cocci (\n            <jats:italic>S. aureus<\/jats:italic>\n            , 100%;\n            <jats:italic>S. epidermidis<\/jats:italic>\n            , 97.2%;\n            <jats:italic>Enterococcus faecalis\/Enterococcus faecium<\/jats:italic>\n            , 96.6%; and\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            , 100%). The specificities of the test modules ranged from 92.5 to 100% for gram-negative bacilli and 97.7 to 100% for gram-positive cocci (\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , 92.5%;\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            , 98.5%;\n            <jats:italic>Klebsiella<\/jats:italic>\n            spp., 100%;\n            <jats:italic>Enterobacter aerogenes<\/jats:italic>\n            , 100%;\n            <jats:italic>S. aureus<\/jats:italic>\n            , 100%,\n            <jats:italic>S. epidermidis<\/jats:italic>\n            , 97.7%;\n            <jats:italic>Enterococcus faecalis\/Enterococcus faecium<\/jats:italic>\n            , 99.6%;\n            <jats:italic>Streptococcus pyogenes<\/jats:italic>\n            , 100%; and\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            , 99.3%). The result of the\n            <jats:italic>mecA<\/jats:italic>\n            gene detection module correlated with the result of the phenotypic oxacillin resistance testing in all 38 isolates of\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            investigated. In conclusion, the Hyplex BloodScreen PCR-ELISA system is well suited for the direct and specific identification of the most common pathogenic bacteria and the direct detection of the\n            <jats:italic>mecA<\/jats:italic>\n            gene of\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            in positive blood cultures.\n          <\/jats:p>","DOI":"10.1128\/jcm.42.7.3147-3152.2004","type":"journal-article","created":{"date-parts":[[2004,7,8]],"date-time":"2004-07-08T18:19:31Z","timestamp":1089310771000},"page":"3147-3152","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Evaluation of the Hyplex BloodScreen Multiplex PCR-Enzyme-Linked Immunosorbent Assay System for Direct Identification of Gram-Positive Cocci and Gram-Negative Bacilli from Positive Blood Cultures"],"prefix":"10.1128","volume":"42","author":[{"given":"Nele","family":"Wellinghausen","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Hygiene, University of Ulm, Ulm"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beate","family":"Wirths","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Hygiene, University of Ulm, Ulm"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Essig","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Hygiene, University of Ulm, Ulm"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Wassill","sequence":"additional","affiliation":[{"name":"Amplex Diagnostics GmbH, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.7-11.2004"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/106.5.600"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.781"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3790-3800.2003"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.10.2193-2196.1991"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.8.2961-2963.2001"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1992.tb00765.x"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199209000-00014"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.814-817.2000"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.7.2574-2578.2000"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.830-838.2000"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1997.tb14815.x"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4705-4707.2003"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00174-3"},{"key":"e_1_3_1_16_2","first-page":"96","volume":"113","year":"2000","unstructured":"Nicholls, T. 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