{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:12:35Z","timestamp":1789863155675,"version":"4.0.1"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso.\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            was sought by detecting several meningococcus-specific genes (\n            <jats:italic>crgA<\/jats:italic>\n            ,\n            <jats:italic>ctrA<\/jats:italic>\n            , 16S rRNA, and\n            <jats:italic>porA<\/jats:italic>\n            ). The PCR-based nonculture method for the detection of\n            <jats:italic>N. meningitidis<\/jats:italic>\n            gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7%, respectively. Most of the laboratories also performed genogrouping assays (\n            <jats:italic>siaD<\/jats:italic>\n            and\n            <jats:italic>mynB<\/jats:italic>\n            \/\n            <jats:italic>sacC<\/jats:italic>\n            ). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.\n          <\/jats:p>","DOI":"10.1128\/jcm.43.1.144-149.2005","type":"journal-article","created":{"date-parts":[[2005,1,5]],"date-time":"2005-01-05T20:14:18Z","timestamp":1104956058000},"page":"144-149","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":82,"title":["Interlaboratory Comparison of PCR-Based Identification and Genogrouping of\n            <i>Neisseria meningitidis<\/i>"],"prefix":"10.1128","volume":"43","author":[{"given":"Muhamed-Kheir","family":"Taha","sequence":"first","affiliation":[{"name":"Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Alonso","sequence":"additional","affiliation":[{"name":"Neisseria Unit and the French National 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