{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:16:41Z","timestamp":1761128201658},"reference-count":29,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Osteoarticular tuberculosis (OAT) is an extrapulmonary tuberculosis and accounts for 1 to 3% of all tuberculosis cases. We used an\n            <jats:italic>rpoB<\/jats:italic>\n            PCR-plasmid TA cloning-sequencing method to detect and identify tubercle bacilli in surgical specimens from patients suspected of having OAT. By comparing the similarities of the\n            <jats:italic>rpoB<\/jats:italic>\n            sequences determined with those in GenBank,\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            was detected in 23 of 43 samples. Three of the 23 positive samples had mutations at codon 531, which are commonly observed in rifampin-resistant\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            strains. Our results suggest that the\n            <jats:italic>rpoB<\/jats:italic>\n            PCR-TA cloning-sequencing method developed, which detects\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            and which simultaneously determines its rifampin susceptibility, can also be used efficiently for the diagnosis of OAT.\n          <\/jats:p>","DOI":"10.1128\/jcm.43.1.174-178.2005","type":"journal-article","created":{"date-parts":[[2005,1,5]],"date-time":"2005-01-05T20:14:18Z","timestamp":1104956058000},"page":"174-178","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Detection and Identification of\n            <i>Mycobacterium tuberculosis<\/i>\n            in Joint Biopsy Specimens by\n            <i>rpoB<\/i>\n            PCR Cloning and Sequencing"],"prefix":"10.1128","volume":"43","author":[{"given":"Yeo-Jun","family":"Yun","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keun-Hwa","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Haihua","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoon-Jong","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bum-Joon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Soonchunhyang University Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goo-Hyun","family":"Baek","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Seoul National University College of Medicine, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee-Joong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Seoul National University College of Medicine, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moon-Sang","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Seoul National University College of Medicine, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myung-Chul","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Seoul National University College of Medicine, Seoul, 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