{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T03:15:16Z","timestamp":1793330116760,"version":"4.5.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2005,2,1]],"date-time":"2005-02-01T00:00:00Z","timestamp":1107216000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Leptospira interrogans<\/jats:italic>\n            sensu stricto is responsible for the most frequent and severe cases of human leptospirosis. The epidemiology and clinical features of leptospirosis are usually associated with the serovars and serogroups of\n            <jats:italic>Leptospira<\/jats:italic>\n            . Because of the difficulties associated with serological identification of\n            <jats:italic>Leptospira<\/jats:italic>\n            strains, we evaluated a novel PCR-based method for typing\n            <jats:italic>L. interrogans<\/jats:italic>\n            serovars. Based upon the genome sequence of\n            <jats:italic>L. interrogans<\/jats:italic>\n            serovar Lai type strain 5660, 44 loci were analyzed by PCR for their variability in size due to the presence of variable-number tandem repeats (VNTR). Seven VNTR loci were found to be powerful markers for serovar identification, epidemiology, and phylogenetic studies of\n            <jats:italic>L. interrogans<\/jats:italic>\n            . This rapid and easy method should greatly contribute to a better knowledge of the epidemiology of\n            <jats:italic>Leptospira<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.43.2.539-545.2005","type":"journal-article","created":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T21:42:56Z","timestamp":1107553376000},"page":"539-545","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":96,"title":["Identification of Variable-Number Tandem-Repeat Loci in\n            <i>Leptospira interrogans<\/i>\n            Sensu Stricto"],"prefix":"10.1128","volume":"43","author":[{"given":"Z.","family":"Majed","sequence":"first","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Bellenger","sequence":"additional","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Postic","sequence":"additional","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Pourcel","sequence":"additional","affiliation":[{"name":"Laboratoire GPMS, Institut de Ge\u0301ne\u0301tique et Microbiologie, Universite\u0301 Paris-Sud, Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Baranton","sequence":"additional","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Picardeau","sequence":"additional","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.2.573"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-2-839"},{"key":"e_1_3_2_4_2","first-page":"4","volume":"12","year":"2004","unstructured":"Denoeud, F., and G. 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